The impact of photobiomodulation therapy on the survival and differentiation of periodontal ligament mesenchymal stem cells exposed to zoledronic acid.

This study assessed the effects of photobiomodulation therapy (PBMT) with different laser wavelengths on the viability and differentiation of periodontal ligament mesenchymal stem cells (PDLMSCs) exposed to zoledronic acid (ZA). In this in vitro study, PDLMSCs were cultured with 5 µM ZA for 48 h and randomly assigned to 6 groups of positive and negative controls, and PBMT with 635 nm (2 and 4 J/cm) and 980 nm (2 and 4 J/cm) lasers. Cell viability was assessed by the methyl thiazolyl tetrazolium (MTT) assay after 24 and 72 h, and mineralized nodule formation was evaluated by Alizarin red staining.

Photobiomodulation effects of 808 nm and 915 nm diode lasers on proliferation and osteogenic differentiation of Buccal fat pad stem cells.

Aim: Photobiomodulation therapy (PBM) is a promising adjunct in tissue engineering due to its ability to stimulate cell proliferation and differentiation. This study evaluated the effects of PBM on buccal fat pad-derived stem cells (BFPSCs) using two diode laser wavelengths.

Methods: BFPSCs were divided into seven groups: six received laser irradiation and one served as control.

The photobiomodulation effects of continuous and pulsed blue diode laser on proliferation and osteogenic differentiation of periodontal ligament stem cells.

This study investigated the photobiomodulation effect of pulsed and continuous blue diode laser on osteogenic differentiation and proliferation of periodontal ligament mesenchymal stem cells. Periodontal Ligament Stem cells were seeded in 96-well plates, and 450 nm blue laser irradiation procedure was performed a day after cell seeding. Each experimental group was divided into two subgroups according to their energy density and irradiation duration: Continuous wave (100 mW, 10s, 2 J/cm and 100 mW, 20 s, 4 J/cm) and pulse wave (200 mW, 10 s, 2 J/cm and 200 mW, 20 s, 4 J/cm and duty cycle 50% for both).

In vitro efficacy of photodynamic therapy with toluidine blue for decontamination of resorbable membranes inoculated with Streptococcus mutans.

Objectives: This study assessed the efficacy of antimicrobial photodynamic therapy (aPDT) with toluidine blue (TB) for decontamination of resorbable membranes inoculated with Streptococcus mutans (S. mutans).

Materials And Methods: In this in vitro study, Mucoderm and Jason resorbable membranes were cut into 15 pieces each, and were inoculated with S.

Reduction of by Chlorella-Mediated Antimicrobial Photodynamic Therapy.

Nowadays, antimicrobial photodynamic therapy (aPDT) has been introduced as one of the minimally invasive methods for disinfection of the surfaces of dental implants. Being derived from seaweed, Chlorella has been used as a photosensitizer in this study. This study aimed to investigate the impacts of aPDT with Chlorella on the rate of reduction of in vitro.

The effect of Er:YAG laser irradiation with different frequencies on surface decontamination of used healing abutments: a supplementary approach to sterilization.

Reusing healing abutments is common practice among clinicians; however, ensuring complete surface decontamination is crucial to avoid further complications. This study aimed to evaluate the cleaning potential of Er: YAG laser at different frequencies, as an adjunctive step prior to autoclave sterilization. Forty contaminated healing abutments were divided into four groups.

Investigating the Effect of Photobiomodulation Therapy With Different Wavelengths of Diode Lasers on the Proliferation and Adhesion of Human Gingival Fibroblast Cells to a Collagen Membrane: An In Vitro Study.

Photobiomodulation (PBM) is considered a promising adjunctive approach in regenerative medicine. This study aimed to investigate the proliferation and adhesion of human gingival fibroblast (HGF) cells to a collagen membrane following PBM. Cultured HGF cells on a collagen membrane received PBM at wavelengths of 808 nm, 915 nm (2 and 4 J/cm), and 660 nm (2.

In Vitro Effect of Photodynamic Therapy With Curcumin in Combination With Photobiomodulation Therapy by 660 nm on the Viability of Human Gingival Fibroblasts.

This study aimed to assess the effect of repeated irradiations of 660 nm photobiomodulation therapy (PBMT) after photodynamic therapy (PDT) with curcumin on the viability of human gingival fibroblasts (HGFs). In this in vitro, experimental study, HGFs were cultured and assigned to five groups: One control group with no intervention and four experimental groups of PDT with curcumin, PBMT (660 nm laser irradiation) immediately after PDT, PBMT immediately and 24 hours after PDT and PBMT immediately and 24 hours and 48 hours after PDT. Cell viability was assessed after 1, 4, and 7 days using the methyl thiazolyl tetrazolium (MTT) assay.

Effect of photobiomodulation therapy with 660 and 980 nm diode lasers on differentiation of periodontal ligament mesenchymal stem cells.

This study aimed to compare the effects of photobiomodulation therapy (PBMT) with 660 and 980 nm diode lasers on differentiation of periodontal ligament mesenchymal stem cells (PDLMSCs). In this in vitro, experimental study, PDLMSCs were obtained from the Iranian Genetic Bank and cultured in osteogenic medium. They were then subjected to irradiation of 660 and 980 nm diode lasers, and their viability was assessed after one, two, and three irradiation cycles using the methyl thiazolyl tetrazolium (MTT) assay.

Temperature Rise and Pain Following the Use of 810 and 980 nm Diode Lasers for Second-Stage Dental Implant Surgery: A Clinical Trial.

Many surgical procedures in a soft tissue are performed using diodes lasers. This study aimed to assess the temperature rise and pain following the use of 810 and 980 nm diode lasers for second-stage dental implant surgery. This clinical trial was conducted on 24 osseointegrated dental implants that were randomly divided into two groups of 810 nm and 980 nm diode lasers.

A comparative evaluation of the effects of 635 nm laser on cell proliferation and osteogenic differentiation of buccal fat pad mesenchymal stem cells.

The purpose of this study was to evaluate the effects of 635 nm diode laser with different powers on undifferentiated mesenchymal stem cells obtained from buccal fat pad. Human buccal fat stem cells were cultured in DMEM containing 10% FBS, penicillin, and streptomycin under 5% CO and 95% humidity. Cells were cultured in 96-well plate and 24 h later, laser irradiation with 635 nm diode laser was performed in four groups of 200, 300, 400, and 500 mW powers in addition to the control group with the same energy density of 4 J/cm.

In Vitro Photobiomodulation Effects of Blue and Red Diode Lasers on Proliferation and Differentiation of Periodontal Ligament Mesenchymal Stem Cells.

This study aimed to assess the photobiomodulation effects of blue and red lasers on the proliferation and osteogenic differentiation of periodontal ligament mesenchymal stem cells (PDLMSCs). PDLMSCs were cultured and tested in 4 groups. The first two groups were exposed to 445 nm diode laser irradiation (200 mW, 6 and 12 J/cm ), and the third group was exposed to 660 nm diode laser irradiation (50 mW, 4 J/cm ).

Surgical reconstruction of peri-implantitis with adjunctive antimicrobial photodynamic therapy: A case report with 5-year follow-up.

Background: Peri-implantitis poses a significant challenge in dental implantology due to its potential to result in the loss of supporting tissue around dental implants. Surgical reconstruction is often recommended for intrabony defects, accompanied by various adjunctive therapies, such as antimicrobial photodynamic therapy (aPDT), for bacterial decontamination. However, the long-term efficacy of such treatments remains unclear.

Evaluation of the effect of photodynamic therapy with Curcumin and Riboflavin on implant surface contaminated with Aggregatibacter actinomycetemcomitans.

Background: Peri-implantitis is a destructive inflammatory disease affecting both hard and soft tissues of the osseointegrated implant and causing bone loss and envelope surrounding the implant. The study aimed at evaluating the effect of Photodynamic therapy with Curcumin and Riboflavin on the level of decontamination of implant surface impregnated with Aggregatibacter actinomycetemcomitans (A.a) biofilm.

The influence of different mode of power density during antimicrobial photodynamic therapy for photokilling of Streptococcus mutans.

Background: The aim of this study was to evaluate the inactivation potency of riboflavin and curcumin plus blue diode laser against Streptococcus mutans with different power densities.

Materials And Methods: In this in vitro study, standard-strain S. mutans was exposed to curcumin and riboflavin plus blue diode laser with different power densities (0.

In Vitro Efficacy of Antimicrobial Photodynamic Therapy With Phycocyanin and Diode Laser for the Reduction of .

Implant surface disinfection is the most difficult phases in treatment of peri-implantitis. This study sought to assess the efficacy of antimicrobial photodynamic therapy (aPDT) with phycocyanin and diode laser for the reduction of . In this in vitro, experimental study, first the minimum inhibitory concentration (MIC) of phycocyanin, the sub-lethal exposure time of the diode laser, and the sub-lethal dose of aPDT were determined.

Comparison of shear bond strength of rebonded brackets prepared with different methods of resin removal: an in vitro study.

Clinicians and researchers have always made every effort to achieve proper bonding between the surface of the tooth and the rebonded orthodontic brackets in order to prevent the re-fracture failure of orthodontic pressures throughout treatment. The aim of this study was to determine the bond strength of rebonded brackets by four adhesive removal methods.

Bacterial Colonization on Different Suture Materials Used in Oral Implantology: A Randomized Clinical Trial.

This study aimed to compare the colonization of (), (), () and () isolated from the oral cavity on different suture materials used in oral implantology. Patients scheduled for implant surgery were included in this study. After flap approximation, the surgical site was sutured using silk, nylon, polyglactin 910 (Vicryl) and triclosan-coated polyglactin 910 (Vicryl Plus) sutures in a randomized order.

Effects of Photobiomodulation Therapy with Various Laser Wavelengths on Proliferation of Human Periodontal Ligament Mesenchymal Stem Cells.

Several methods have been proposed to enhance the regeneration and healing time in periodontal therapy. Photobiomodulation therapy (PBMT) is a recently suggested novel technique for this purpose. This study aimed to compare the efficacy of PBMT with various laser wavelengths and energy densities on proliferation of human periodontal ligament mesenchymal stem cells (PDLMSCs).

Effect of Different Energy Densities of 915 nm Low Power Laser on the Biological Behavior of Human Gingival Fibroblast Cells In Vitro.

Photobiomodulation is recognized as an effective method for adjunct therapy in periodontal treatments. Our purpose in this study was to investigate the effects of different energy densities of 915 nm diode laser on the viability and viability capacity of human gingival fibroblast cells. Cell samples were examined in five groups, including four irradiation groups with low-level diode laser 915 nm, 1, 2, 3, 4 J cm and a control group (no Laser irradiation).

Efficacy of titanium brush, 915 nm diode laser, citric acid for eradication of Staphylococcus aureus from implant surfaces.

Background: This study aimed to assess the efficacy of titanium brush, 915 nm diode laser, citric acid and the combination of latter two with titanium brush for decontamination of SLA surface mini-implants.

Methods: Seventy-five mini-implants contaminated with Staphylococcus aureus (S. aureus) were randomly divided into five experimental groups (n = 12) of titanium brush (TiB), laser, citric acid (CA), brush-laser, and brush-acid, positive [n = 12; chlorhexidine mouthwash (CHX)] and negative [n = 2; phosphate buffered saline (PBS)] control groups and one no-treatment group (n = 1).

Blue Light Photodynamic Therapy With Curcumin and Riboflavin in the Management of Periodontitis: A Systematic Review.

The aim of this article was to evaluate reports in the scientific literature that used antimicrobial photodynamic therapy (aPDT) with a blue light source and curcumin and riboflavin as photosensitizers in the management of periodontitis. The search was conducted in electronic databases, including PubMed, Web of Science, and Scopus, with the keywords "photodynamic therapy", "antimicrobial photodynamic therapy", "laser activated disinfection", "photoactivated disinfection", "light activated disinfection" "LED", "Periodontitis", "Curcumin", "Riboflavin", and "periodontitis" from 2012 to 2020. After evaluating a total of 24 relevant articles, 13 articles were selected, full texts were read, and the data were extracted and placed in a table.

In Vitro Effect of Photodynamic Therapy with Indocyanine Green Followed by 660 nm Photobiomodulation Therapy on Fibroblast Viability.

This in vitro study sought to assess the effect of repetitive PBMT on the viability of fibroblasts following aPDT with indocyanine green (ICG). In this in vitro experimental study, human gingival fibroblasts (HGFs) were obtained and incubated in a culture medium. After reaching 10 000 cells cm , the cells were divided into five groups of control, aPDT with ICG and 808 nm (energy density of 24 J cm ), PBMT immediately after aPDT, PBMT with 660 nm (energy density of 7.

Photobiomodulation Effect of Different Diode Wavelengths on the Proliferation of Human Gingival Fibroblast Cells.

This study is focused on comparing the effect of various energy densities and wavelengths of diode lasers on the proliferation of human gingival fibroblast (HGF) cells in vitro. In this study, 204 sample cells were examined in 4 test groups (laser radiation) and 1 control group (non-laser radiation). The proliferation rate of radiated cells with wavelengths of 635, 660, 808 and 980 nm and the densities of 1, 1.

Cytotoxicity and Antibacterial Activity of Toothpastes and Mouthwashes Available in the Iranian Market.

Toothpastes and mouthwashes contain chemicals that may be harmful to oral tissues. This study assessed the cytotoxicity and antibacterial activity of toothpastes and compare the Iranian and foreign toothpastes and mouthwashes available in the Iranian market in this respect. Twenty samples (13 toothpastes and 4 mouthwashes) were selected.