Nanofibrous GelMA-Based Scaffolds Support Human Adipose-Derived Mesenchymal Stem/Stromal Cell Adhesion, Viability, and Growth.

Drawing inspiration from the extracellular matrix (ECM), where fiber organization profoundly influences cell behavior, electrospun scaffolds have emerged as powerful tools for modulating cellular responses in vitro. While electrospinning enables the replication of ECM architecture, selecting suitable materials is paramount for effective cell adhesion and growth. In this study, we aimed to develop cost-effective scaffolds for human adipose-derived mesenchymal stromal cells (hAD-MSCs) using gelatin methacrylate (GelMA) blended with either polycaprolactone (PCL) or poly(ethylene glycol) dimethacrylate (PEGDMA).

Mimicking the extracellular world: from natural to fully synthetic matrices utilizing supramolecular biomaterials.

The extracellular matrix (ECM) has evolved around complex covalent and non-covalent interactions to create impressive function-from cellular signaling to constant remodeling. A major challenge in the biomedical field is the design and control of synthetic ECMs for applications ranging from tissue engineering to neuromodulation to bioelectronics. As we move towards recreating the ECM's complexity in hydrogels, the field has taken several approaches to recapitulate the main important features of the native ECM ( mechanical, bioactive and dynamic properties).

Digital Light Processing Resins with Programmable Shape Memory for Biomedical Applications.

The creation of biodegradable and biocompatible shape memory polymers amenable to biofabrication techniques remains a challenge. The ability to create shape-changing biodegradable objects that are triggered at body temperature opens up possibilities in tissue engineering, minimally invasive surgery, and actuating bioimplants. Merging Digital Light Processing (DLP) printing with shape memory polymers brings us closer to new smart biomedical outcomes.

Tailoring Network Topology in Mechanically Robust Hydrogels for 3D Printing and Injection.

Tissue engineering and regenerative medicine are confronted with a persistent challenge: the urgent demand for robust, load-bearing, and biocompatible scaffolds that can effectively endure substantial deformation. Given that inadequate mechanical performance is typically rooted in structural deficiencies─specifically, the absence of energy dissipation mechanisms and network uniformity─a crucial step toward solving this problem is generating synthetic approaches that enable exquisite control over network architecture. This work systematically explores structure-property relationships in poly(ethylene glycol)-based hydrogels constructed utilizing thiol-yne chemistry.

Molecular Tuning of a Benzene-1,3,5-Tricarboxamide Supramolecular Fibrous Hydrogel Enables Control over Viscoelasticity and Creates Tunable ECM-Mimetic Hydrogels and Bioinks.

Traditional synthetic covalent hydrogels lack the native tissue dynamics and hierarchical fibrous structure found in the extracellular matrix (ECM). These dynamics and fibrous nanostructures are imperative in obtaining the correct cell/material interactions. Consequently, the challenge to engineer functional dynamics in a fibrous hydrogel and recapitulate native ECM properties remains a bottle-neck to biomimetic hydrogel environments.

Human adipose mesenchymal stromal cells growing into PCL-nHA electrospun scaffolds undergo hypoxia adaptive ultrastructural changes.

Human Adipose-Derived Mesenchymal Stem/Stromal Cells (hAD-MSCs) have great potential for tissue regeneration. Since transplanted hAD-MSCs are likely to be placed in a hypoxic environment, culturing the cells under hypoxic conditions might improve their post-transplantation survival and regenerative performance. The combination of hAD-MSCs and PCL-nHA nanofibers synergically improves the contribution of both components for osteoblast differentiation.

Trends in Double Networks as Bioprintable and Injectable Hydrogel Scaffolds for Tissue Regeneration.

Additive manufacturing and injection are essential tools in the rapidly developing field of personalized medicine and are particularly promising for applications in regenerative medicine. One of the biggest challenges in this vibrant research domain remains the processing of complex formulations with robust mechanical properties. Mimicking the native extracellular matrix associated with many tissues requires materials that have high degrees of functionality for performing the complex array of functions within the cellular environment.

14-3-3ε protein-loaded 3D hydrogels favor osteogenesis.

3D printing has emerged as vanguard technique of biofabrication to assemble cells, biomaterials and biomolecules in a spatially controlled manner to reproduce native tissues. In this work, gelatin methacrylate (GelMA)/alginate hydrogel scaffolds were obtained by 3D printing and 14-3-3ε protein was encapsulated in the hydrogel to induce osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASC). GelMA/alginate-based grid-like structures were printed and remained stable upon photo-crosslinking.

Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features.

The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features.

Bioadhesive and biocompatible films as wound dressing materials based on a novel dendronized chitosan loaded with ciprofloxacin.

The bioadhesive polymeric films as topical drug delivery systems are interesting alternatives to improve the pharmacotherapy and patient compliances. New derivate biomaterials based on weisocyanate- dendronized PVP- crosslinked chitosan and loaded with ciprofloxacin (CIP), as model drug, were used to prepare bioadhesive films. Relevant in vitro/in vivo attributes to define main physicochemical and biopharmaceutical characteristics for topical wound-healing applications were evaluated.

Current advances in electrospun gelatin-based scaffolds for tissue engineering applications.

The development of biomimetic highly-porous scaffolds is essential for successful tissue engineering. Electrospun nanofibers are highly versatile platforms for a broad range of applications in different research areas. In the biomedical field, micro/nanoscale fibrous structures have gained great interest for wound dressings, drug delivery systems, soft and hard-tissue engineering scaffolds, enzyme immobilization, among other healthcare applications.