Publications by authors named "Alexis D Corrado"
Glioblastoma (GBM) is the most common and aggressive primary brain malignancy. Adhesion G protein-coupled receptors (aGPCRs) have attracted interest for their potential as treatment targets. Here, we show that CD97 (ADGRE5) is the most promising aGPCR target in GBM, by virtue of its de novo expression compared to healthy brain tissue.
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- Antibody responses are crucial for defending against SARS-CoV-2 by stopping the virus from entering cells, and a new assay called 2D-MBBA has been developed to measure various antibody isotypes simultaneously.
- This assay was used to analyze IgG, IgM, and IgA levels against the spike protein and its variants, and machine learning significantly improved predictions of how well these antibodies neutralize the virus in convalescent patients.
- The method can differentiate between antibody profiles in convalescent and vaccinated individuals and offers the potential for rapid testing of neutralization efficacy against new variants and pathogens using just a small blood sample.
Congenital myasthenia (CM) is a devastating neuromuscular disease, and mutations in DOK7, an adaptor protein that is crucial for forming and maintaining neuromuscular synapses, are a major cause of CM. The most common disease-causing mutation (DOK7) truncates DOK7 and leads to the loss of two tyrosine residues that are phosphorylated and recruit CRK proteins, which are important for anchoring acetylcholine receptors at synapses. Here we describe a mouse model of this common form of CM (Dok7 mice) and a mouse with point mutations in the two tyrosine residues (Dok7).
View Article and Find Full Text PDFMultiplex bead binding assays using off-the-shelf components and common flow cytometers.
J Immunol Methods
March 2021
Article Synopsis
- The study focuses on developing a cost-effective multiplex bead binding assay (MBBA) for analyzing protein-ligand interactions without the need for specialized equipment or expensive reagents.
- The method innovatively uses biotin-streptavidin interactions and fluorescently labeled magnetic beads to enhance experimental throughput.
- The effectiveness of this new MBBA method is demonstrated by characterizing antibodies against SARS-CoV-2, significantly improving efficiency and reducing the amount of antigen required compared to traditional methods.
Fluoride ion channels of the Fluc family selectively export F ions to rescue unicellular organisms from acute F toxicity. Crystal structures of bacterial Fluc channels in complex with synthetic monobodies, fibronectin-derived soluble β-sandwich fold proteins, show 2-fold symmetric homodimers with an antiparallel transmembrane topology. Monobodies also block Fluc F current via a pore blocking mechanism.
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