Naphthalimide-organometallic hybrids as multi-targeted anticancer and luminescent cellular imaging agents.

1,8-Naphthalimides with an organometallic moiety with anticancer and luminescence or photoactive properties are reviewed. Primarily highlighted are 1,8-naphthalimide complexes with iron, platinum, and ruthenium, while honourable mentions are given to iridium, rhenium and rhodium complexes. The cytotoxicity, mechanism of action and cell selectivity of the compounds are discussed alongside their photophysical properties for monitoring interactions with biomolecules, most notably DNA, by absorption, fluorescence and dichroism spectroscopy.

Expanding Targeted Instrumentation for Discovery Applications: Complement Reporter Ion Quantification with a Quadrupole-Ion Trap Instrument.

Proteomic workflows have traditionally been divided into discovery-based and targeted approaches with instrumentation optimized specifically for each. Discovery experiments typically utilize high-resolution analyzers, while targeted workflows rely on the sensitivity and specificity of triple quadrupole systems. Recently, a quadrupole-ion trap instrument (Stellar MS) has demonstrated superior performance for targeted applications compared to conventional triple quadrupoles.

Accelerating Characterization of Therapeutic Antibodies: A Comparative Assessment of icIEF-UV/MS and the Traditional Fractionation Workflow.

Accurate characterization of charge variants in biologics is crucial to uphold stringent quality standards ensuring the safety and efficacy of biotherapeutic products and regulatory requirements for the Biologic Licensing Application (BLA) process enabling marketing application. These variants, arising from post-translational modifications (PTMs) during upstream processing and enzymatic/nonenzymatic reactions in downstream processing and storage, can significantly impact therapeutic potency, efficacy, and immunogenicity. Conventional methods for characterizing charge variants typically involve labor-intensive fraction enrichment, consuming time and resources.

Wireless arm-worn bioimpedance sensor for continuous assessment of whole-body hydration.

Assessment of whole-body hydration (WBH) is crucial for health management and disease diagnosis. Traditional methods are invasive or require bulky equipment, making them impractical for mobile, continuous sensing. We present a wearable bioimpedance sensor with strategic electrode placement across the arm for noninvasive, continuous, and mobile WBH monitoring.

The hidden asymmetry: facet joint tropism as a clue to spinal malalignment and muscle degeneration in adult spinal deformity.

Objectives: The role of facet joint tropism (FJT) in degenerative spinal disorders such as disc herniation, spondylolisthesis, and lumbar canal stenosis is well-established. However, its association with adult spinal deformity (ASD) remains underexplored. Hence, we aim to study the correlation of FJT with spinopelvic parameters and lumbar paraspinal muscle morphology in ASD patients.

The Nature-Based Paradigm Shift: Identifying and Overcoming Barriers to Sustainable Water Quality Solutions.

This study investigates the barriers and opportunities for implementing nature-based solutions to improve water quality in the United States, utilizing a mixed-methods approach. Data were collected through key informant interviews ( = 27), focus group discussions, and an online survey ( = 167). The triangulation of these methods provided a comprehensive understanding of stakeholder perspectives across various sectors including water treatment plant managers, government officials, regulators, and landowners.

A patient-centered qualitative evaluation of meaningful change on the NSAA and PUL in Duchenne Muscular Dystrophy.

Background: Duchenne Muscular Dystrophy (DMD) is a rare X-linked genetic disorder caused by mutations in the dystrophin gene. The North Star Ambulatory Assessment (NSAA) and Performance of Upper Limb (PUL) are used to measure motor performance in ambulatory and non-ambulatory individuals, respectively. There is limited published qualitative evidence on what constitutes a meaningful change on either instrument.

Mitigating Wildfire Impact on Water Quality through Climate-Based Financing: A Case Study of the Provo River Watershed.

Following wildfires, riverine water quality in forested watersheds is prone to degradation, impacting drinking water treatment and potentially causing increased carbon emissions because of additional electricity consumption during treatment. We explore the potential for climate-based financing to support wildfire mitigation and watershed restoration by reducing potential water treatment energy demand following a fire within the Provo River watershed, Utah, USA. We model pre- and post-wildfire erosion and water quality in the Provo River using GeoWEPP.

Sensitive and Accurate Proteome Profiling of Embryogenesis Using Real-Time Search and TMTproC Quantification.

Multiplexed proteomics has become a powerful tool for investigating biological systems. Using balancer-peptide conjugates (e.g.

Perioperative Complications of Oblique Lumbar Interbody Fusion (OLIF): 5 Years of Experience with OLIF.

 The objective of this study was to share our early experience with oblique lumbar interbody fusion (OLIF), with emphasis on the perioperative complications and determine clinical outcomes following OLIF.  It was a retrospective prospective study performed at a single institute from March 2019 to August 2023. A total of 56 consecutive patients who had undergone OLIF for degenerative spine disorders were enrolled in the study.

Novel Technique to Close Stellate Corneal Lacerations.

Purpose: The study was performed to evaluate and optimize a novel technique to close stellate corneal lacerations in a wet laboratory environment.

Materials And Methods: Twenty-one ex vivo porcine eyes with standardized, experimentally created stellate corneal lacerations were assigned to one of three surgical repair techniques. In Group 1 (n = 9), lacerations were closed with simple interrupted sutures only.

Socratic Artificial Intelligence Learning (SAIL): The Role of a Virtual Voice Assistant in Learning Orthopedic Knowledge.

Objective: We hypothesized that learning through multiple sensory modalities would improve knowledge recall and recognition in orthopedic surgery residents and medical students.

Design: We developed a virtual study assistant, named Socratic Artificial Intelligence Learning (SAIL), based on a custom-built natural language processing algorithm. SAIL draws from practice questions approved by the American Board of Orthopaedic Surgery and quizzes users through a conversational, voice-enabled Web interface.

ERK synchronizes embryonic cleavages in Drosophila.

Extracellular-signal-regulated kinase (ERK) signaling controls development and homeostasis and is genetically deregulated in human diseases, including neurocognitive disorders and cancers. Although the list of ERK functions is vast and steadily growing, the full spectrum of processes controlled by any specific ERK activation event remains unknown. Here, we show how ERK functions can be systematically identified using targeted perturbations and global readouts of ERK activation.

Global protein turnover quantification in Escherichia coli reveals cytoplasmic recycling under nitrogen limitation.

Protein turnover is critical for proteostasis, but turnover quantification is challenging, and even in well-studied E. coli, proteome-wide measurements remain scarce. Here, we quantify the turnover rates of ~3200 E.

Decarbonizing Water: The Potential to Apply the Voluntary Carbon Market toward Global Water Security.

The increase in global water insecurity is one of the first perceivable effects of climate change. Two billion people are now without access to safe drinking water, and four billion experience water stress at least once a year, primarily in low per-capita emission countries. This nexus between climate change and water insecurity has significant implications for the global economy, with the water sector contributing 10% of global emissions.

Structure and assembly of a bacterial gasdermin pore.

In response to pathogen infection, gasdermin (GSDM) proteins form membrane pores that induce a host cell death process called pyroptosis. Studies of human and mouse GSDM pores have revealed the functions and architectures of assemblies comprising 24 to 33 protomers, but the mechanism and evolutionary origin of membrane targeting and GSDM pore formation remain unknown. Here we determine a structure of a bacterial GSDM (bGSDM) pore and define a conserved mechanism of pore assembly.

Spontaneous Unilateral Hyphema in a Newborn With Occiput Posterior Presentation: A Case Report.

Few cases of isolated spontaneous hyphema in the newborn have been reported. A case of a term vaginally delivered female newborn who was diagnosed as having a hyphema in the left eye 18 hours after birth is presented. Delivery was complicated with fetal head malposition and the delivery was prolonged.

Framboesiform Facial Lesions and Chorioretinopathy as Presenting Signs of Syphilis in an Immunocompetent Patient.

Syphilis has long been considered the "great masquerader," notorious for its varying presentations and ability to affect most organ systems in the body. We report the case of a 41-year-old immunocompetent man who presented to ophthalmology with rapidly progressive visual complaints from bilateral panuveitis and concomitant verrucous facial lesions initially disregarded by the patient as acne. Serum testing for syphilis was positive, and he was admitted for 14 days of intravenous (IV) penicillin with multiservice care from dermatology, ophthalmology, and infectious disease.

Structural basis of Gabija anti-phage defence and viral immune evasion.

Bacteria encode hundreds of diverse defence systems that protect them from viral infection and inhibit phage propagation. Gabija is one of the most prevalent anti-phage defence systems, occurring in more than 15% of all sequenced bacterial and archaeal genomes, but the molecular basis of how Gabija defends cells from viral infection remains poorly understood. Here we use X-ray crystallography and cryo-electron microscopy (cryo-EM) to define how Gabija proteins assemble into a supramolecular complex of around 500 kDa that degrades phage DNA.

Use of FLOSIC for understanding anion-solvent interactions.

An Achille's heel of lower-rung density-functional approximations is that the highest-occupied-molecular-orbital energy levels of anions, known to be stable or metastable in nature, are often found to be positive in the worst case or above the lowest-unoccupied-molecular-orbital levels on neighboring complexes that are not expected to accept charge. A trianionic example, [Cr(C2O4)3]3-, is of interest for constraining models linking Cr isotope ratios in rock samples to oxygen levels in Earth's atmosphere over geological timescales. Here we describe how crowd sourcing can be used to carry out self-consistent Fermi-Löwdin-Orbital-Self-Interaction corrected calculations (FLOSIC) on this trianion in solution.

Brief tele-mindfulness-based intervention: A multicenter randomized controlled trial.

Background: Coronavirus disease 2019 (COVID-19) has proven to be detrimental to the psychological well-being of healthcare providers (HCP). This study was a psychological intervention during the COVID-19 pandemic to check extent to which brief mindfulness-based interventions (MBIs) and progressive muscle relaxation (PMR) affect psychological well-being, resilience, and anxiety of HCPs.

Materials And Methods: A randomized trial study conducted from July to August 2020.

Structural homology screens reveal host-derived poxvirus protein families impacting inflammasome activity.

Viruses acquire host genes via horizontal transfer and can express them to manipulate host biology during infections. Some homologs retain sequence identity, but evolutionary divergence can obscure host origins. We use structural modeling to compare vaccinia virus proteins with metazoan proteomes.