Sunlight-Assisted Photodegradation of Tetracycline Using ZnS-Melamine-Formaldehyde Nanocomposites.

The degradation of colorless tetracycline hydrochloride (TCH), a widely used antibiotic, is a significant environmental concern due to its persistence in aquatic systems. The zinc sulfide (ZnS) nanoparticle fabricated melamine-formaldehyde polymer (MFP)-based nanocomposite (ZnS-MFP) was prepared via a hydrothermal polymerization method, followed by surface modification through a simple precipitation route. The degradation of TCH through photocatalysis adheres to pseudo-first-order kinetics with a significantly faster rate under natural sunlight than under artificial bulb light.

Research Communication: Combination Therapy of Upadacitinib With Infliximab, Risankizumab, Ustekinumab or Vedolizumab for Refractory Crohn's Disease: A Descriptive Case Series.

Combining two advanced therapies may improve outcomes in Crohn's disease (CD) refractory to monotherapy. We conducted a descriptive case series of 27 patients with CD who initiated combination therapy with upadacitinib and infliximab (n = 1), risankizumab (n = 17), ustekinumab (n = 3) or vedolizumab (n = 6). At 12 weeks, 24 achieved clinical response and 9 achieved steroid-free remission.

Structure-guided engineering of CCL27 enhances natural ligand CAR T-cells against CCR10 for multiple myeloma.

Despite the success of BCMA CAR-Ts, many multiple myeloma patients relapse and require additional therapeutic options. Our group previously identified the chemokine receptor CCR10 as a potential alternate target to address this need. Here, we validated CCR10 expression on primary myeloma tumors and sought to develop CAR T-cells against CCR10, utilizing its natural ligand CCL27 as a CAR binding element.

Structural insights into Arylidenehydrazinyl Benzenesulfonamides as potent mycobacterial carbonic anhydrase inhibitors.

Aims: To design, synthesize, and assess novel sulfonamide hydrazone derivatives as selective inhibitors of carbonic anhydrase.

Materials And Methods: Two series of 4-(arylidenehydrazinyl)benzenesulfonamides () and N-arylidene-4-methylbenzenesulfonohydrazides () were synthesized and evaluated against recombinant MtCA isoforms 1 and 3, and human carbonic anhydrase isoforms I and II by enzyme inhibition assays. Molecular docking and molecular dynamics simulations assessed the binding stability and coordination with the active-site zinc ion.

Single-Molecule Studies with AFM and High-Speed AFM: From Nucleosomes to Chromosomes.

Atomic force microscopy (AFM) is a single-molecule imaging technique invented in 1985. Development of high-speed AFM (HS-AFM) in 2001 now allows for the direct visualization of the dynamics and kinetics of biological molecules. In this chapter, we describe methods to both reconstitute in vitro and extract in vivo chromatin for air mode AFM and HS-AFM studies.

Prospective study on outcomes of endotherapy for pancreatic divisum in patients of recurrent acute pancreatitis.

Background And Study Aims: Minor papilla endotherapy success rate is highly variable for pancreatic divisum (PD) among recurrent acute pancreatitis (RAP) patients due to frequent relapses. Therefore, we assessed effectiveness and predictors of successful endotherapy.

Patients And Methods: This was a prospective observational study of patients with RAP who underwent minor papilla sphincterotomy and prophylactic stenting for PD.

A study exploring predictors of cell phone use while walking among adolescents based on theory of planned behavior.

Background: Walking is a complex activity that requires high levels of perception and cognitive abilities. Healthy pedestrians who use mobile phones while walking will have their decision-making process affected to varying degrees and may be at greater risk of injury. Previous studies have shown that using mobile phones while walking is becoming increasingly common.

A Decade of Prenatal Genetic Diagnostics: Insights From 1949 Cases at a Medical Genetics Facility in South India.

Objective: Our objective was to report on the performance of prenatal diagnostic procedures for genetic testing in a developing country.

Method: This retrospective study involved a review of medical records of women who underwent prenatal diagnostic procedures from 2014 to 2024 at a Medical Genetics facility in South India. Data on the type of procedure, indication for testing, types of tests, outcomes, and complications of procedures were recorded and analyzed using descriptive statistics.

Cathodic Exfoliation of Various Graphite Materials in Potassium Chloride Electrolyte.

Cathodic exfoliation of graphite has emerged as an attractive method to synthesize high-quality and lo- defect graphene. Here, it is demonstrated that the type of starting graphite material influences the properties of exfoliated graphene. Graphite foil, natural graphite, and graphite rods were examined in the exfoliation processes performed in 3.

Transient receptor potential vanilloid type 4 channels mediate bladder cancer cell proliferation, migration, and chemoresistance.

Bladder cancer (BLCA) is the second most common urologic cancer in the United States and worldwide and mostly affects the aging population. Despite several ongoing clinical trials, treatment paradigms for BLCA have not changed significantly. Here, we investigated the expression of transient receptor potential vanilloid type 4 (TRPV4) in patients with BLCA and its role in calcium influx, cell proliferation, and migration using normal human urothelial cells and BLCA cells.

Real-World Experience of Upadacitinib Reinduction and High Dose Maintenance Therapy in Inflammatory Bowel Disease.

Background: Upadacitinib (UPA) is approved for the treatment of moderately to severely active Crohn's disease (CD) or ulcerative colitis (UC). Responders to UPA 45mg daily (QD) may lose response (LOR) after transition to lower maintenance doses. We describe our real-world experience of LOR, reinduction, and subsequent maintenance with UPA 45mg QD.

Prevalence and factors associated with insomnia among firefighting personnel in Dhaka division, Bangladesh.

Background: Firefighting is a challenging and stressful job, and firefighters face many adverse conditions while performing their duties. The study aimed to assess the prevalence of insomnia among firefighting staff working in the Dhaka division of Bangladesh and identify the factors contributing to the severity of insomnia.

Methods: A cross-sectional study was conducted among a total of 406 employees of the Department of Fire Service & Civil Defense (FSCD) working in randomly selected nine districts of the Dhaka division using a simple random sampling (SRS) technique.

Emergent technologies in clinical neurophysiology to study the central nervous system: IFCN handbook chapter.

This chapter reviews recent breakthroughs in neurophysiological brain mapping, focusing on EEG, MEG, and MRI technologies and their integration with stimulation techniques. High-density and portable EEG systems now allow more precise, user-friendly, and mobile recordings. Machine learning enhances biomarker detection and diagnostic power, particularly in epilepsy, cognitive disorders, and sleep pathology.

Testosterone Suppresses IL-17 Expression by Targeting RORγt Functions.

Th17 cells play a crucial role in autoimmune disease pathogenesis. However, the mechanisms behind the sex differences in immune responses, particularly women's higher susceptibility to autoimmune diseases, remain unclear. This study investigated the role of testosterone in modulating the IL-17 response.

Oncogenic lncRNA transgene transcription modulates epigenetic memory at a naïve chromosomal locus.

Maintaining genome integrity is essential for the proper functioning and development of organisms. An intriguing aspect is that neocentromeres can form at non-centromeric sites. CENP-A, a key epigenetic marker of centromeres, is often mislocalized to ectopic sites in cancers when overexpressed.

A structured approach for identifying disease analogs for pulmonary arterial hypertension.

Drug manufacturers often use disease analogs to describe diseases-particularly rare diseases-to payers, policymakers, and stakeholders. However, these comparisons are typically anecdotal. We propose a 4-step, systematic approach to identify disease analogs based on prespecified metrics and apply it to a rare disease, pulmonary arterial hypertension (PAH), as a case study.

Pre-mRNA Differential 5' Splicing: A Rescue of Functional Protein Properties from Pathogenic Gene Variants and a Lifeline for Fanconi Anemia D1 Patients.

Fanconi anemia (FA) is a DNA repair deficiency disorder associated with genomic and chromosomal instability and a high cancer risk. In a small percentage of cases, FA is caused by biallelic pathogenic variants (PVs) in the gene, defining the FA-D1 subtype. Experimental and epidemiologic data indicate that the complete absence of BRCA2 is incompatible with viability.

A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function.

RNA-binding proteins (RBPs) are key regulators of gene expression; however, their RNA-binding specificities, that is, motifs, have not been comprehensively determined. Here we introduce Eukaryotic Protein-RNA Interactions (EuPRI), a freely available resource of RNA motifs for 34,746 RBPs from 690 eukaryotes. EuPRI includes in vitro binding data for 504 RBPs, including newly collected RNAcompete data for 174 RBPs, along with thousands of predicted motifs.

Persistent Imbalance: Women's Representation in North American Pediatric Cardiology Leadership Roles.

Background: Despite recent gender parity of physicians entering pediatric cardiology, representation of women leaders lags their male colleagues.

Objectives: We sought to better understand the variation in women in leadership roles in pediatric cardiology.

Methods: The gender of physicians in 16 prespecified leadership positions was collected by survey between July 2022 and January 2023 from pediatric cardiology programs with >5 cardiologists in North America.

Factors Associated With Mortality in Leukemia and Lymphoma With COVID-19: A National Inpatient Sample Analysis (2020-2021).

Background Patients with hematological malignancies face a substantially increased mortality from COVID-19. Although the peak of the COVID-19 pandemic has passed, the virus remains common, and understanding its impact on vulnerable groups such as those with hematologic malignancies remains crucial. Limited research exists on mortality patterns in leukemia and lymphoma patients during the pandemic.