Deep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite Protein Sequencing by Mass Spectrometry.

The ability to sequence proteins without reliance on a genomic template defines a critical frontier in modern proteomics. This approach, known as protein sequencing, is essential for applications such as antibody sequencing, microbiome proteomics, and antigen discovery, which require accurate reconstruction of peptide and protein sequences. While trypsin remains the gold-standard protease in proteomics, its restricted cleavage specificity limits peptide diversity.

Characterisation, degradation and regeneration of luminescent Ag clusters in solution.

Luminescent Ag clusters are prepared with lipoic acid (LA) as the ligand. Using a combination of mass spectrometry, optical spectroscopy and analytical ultracentrifugation, the clusters are found to be highly monodisperse with mass 5.6 kDa.