Codon and Signal Peptide Optimization to Enhance Therapeutic Antibody Production from CHO Cells.

A simple and rapid method for reproducible transient expression experiments to quickly identify the best conditions before the development of a stable CHO cell line is described, using adalimumab as a model antibody. Firstly, the signal peptide (SP) is optimized using different in silico programs, of which those having a D score of more than 0.85 will be selected.

Unraveling the structural complexity of and the effect of calcination temperature on calcium phosphates derived from bones.

In this study, the interplay between the structural complexity, microstructure, and mechanical properties of calcium phosphates (CaPs) derived from fish bones, prepared at various calcination temperatures, and their corresponding sintered ceramics was explored. Fourier-transform infrared analysis revealed that the calcined powders primarily consisted of hydroxyapatite (HAp) and carbonated calcium hydroxyapatite, with an increasing concentration of Mg-substituted β-tricalcium phosphate (β-TCP) as the calcination temperature was increased. X-ray diffraction patterns showed enhanced sharpness of the peaks at higher temperatures, indicating a larger crystallite size and improved crystallinity.

Impacts of pre-treatment methods on the morphology, crystal structure, and defects formation of hydroxyapatite extracted from Nile tilapia scales.

The comprehensive control of hydroxyapatite (HAp), involving morphological and structural variations, particle sizes, and defect formations, has garnered considerable attention for its versatile functionalities, rendering it applicable in diverse contexts. This work examined the shape, structure and optical characteristics, and defect formation in hydroxyapatite (HAp) extracted from Nile tilapia () scales with various pre-treatments through experiments and density functional theory (DFT) calculations. Utilizing scanning electron microscopy, our findings revealed that dried fish scales (FS-D) exhibited a layered pattern of collagen fibers, while boiled fish scales (FS-B) had smoother surfaces and significantly reduced collagen content.