OsmoFold: A high-throughput tool for predicting the impact of osmolytes on protein structure.

Small molecules can affect a protein's fold and stability. Physical chemistry conceptualizes this effect using Tanford's transfer model, which describes the change in free energy of a protein upon transfer from water to a solution containing some concentration of a small molecule. Using additive transfer free energies of individual amino acids, one can apply the transfer model to quantitatively predict how small molecules affect protein folding equilibria.