Global Burden of Human Metapneumovirus: Bridging Gaps in Prevention, Diagnostics and Treatment.

Human metapneumovirus (hMPV) is a respiratory virus with a significant impact, particularly on the paediatric population, yet it remains an under-recognized contributor to respiratory infections globally. Recent epidemics in China, India and Europe underscore its clinical burden, with severe cases linked to bronchiolitis, pneumonia and exacerbations of chronic conditions. Despite its impact, hMPV remains underdiagnosed due to limited access to molecular diagnostics and routine surveillance, particularly in low- and middle-income countries.

Patchouli Ethanol Extract Benth. Against Aging Profiles in Doxorubicin-Induced 3T3-L1 Fibroblast Cell Lines.

Objectives: This research aimed to investigate the potential of Benth's ethanolic extract (PCEE) as an antiaging agent.

Methods: We treated 3T3-L1 cells induced by doxorubicin with PCEE and assessed them with antiaging markers such as gene and telomere length with polymerase chain reaction (PCR), or reactive oxygen species (ROS) accumulation with 2,7-dichlorofluorescein diacetate (DCFHDA).

Results: This study demonstrates that PCEE can inhibit ROS accumulation, decrease the expression level of the senescence-associated β-galactosidase (SA-β-gal) and maintain telomere length in doxorubicin-induced 3T3-L1 cells.