The assessment of interaction kinetics between specific monoclonal antibody and immobilized SARS-CoV-2 nucleoprotein.

The emergence of SARS-CoV-2 has posed significant global health challenges. The nucleocapsid protein (N-protein) is a structural part of the SARS-CoV-2 virus and an important immunogenic target of specific antibodies, which are developed in the organism during the infection by this virus. Artificially designed specific (monoclonal and polyclonal) antibodies are also used for therapeutic and bioanalytical purposes, therefore, the assessment and characterization of newly designed antibodies is an important analytical issue.

Tracking changes in anxiety, depression, and stress among Ukrainian university students: a three-wave study from the COVID-19 pandemic to two years of ongoing full-scale war.

Since 2020, modern youth in Ukraine have faced an exceptionally challenging period, first enduring the burdens of the COVID-19 pandemic and then being thrust into the full-scale war, characterized by persistent traumatic exposure. Given the profound and lasting psychological consequences of such crises, it is essential to monitor the long-term impact of war on youth mental health. However, despite the urgency of this issue, longitudinal studies with large, diverse samples remain scarce.

Advanced Electrospun Chitosan-(Polylactic Acid)-(Silver Nanoparticle)-Based Scaffolds for Facilitated Healing of Purulent Wounds: A Preclinical Investigation.

Biomaterials modified by antibacterial substances, including nanoparticles, open new opportunities for the effective treatment of infected wounds. Unfortunately, most publications focused only on experiments in vitro, with limited understanding of their potential for the clinic. This study evaluates the effectiveness in vivo of electrospun chitosan/polylactic acid (Ch/PLA) membranes enriched with silver nanoparticles (AgNPs) for purulent wound treatment.

Advanced biomaterials in tissue engineering: A critical review of nanocomposites based on bacterial cellulose, MXenes, hydroxyapatite, and metal particles for regenerative medicine.

Soft and hard tissues have limited regenerative potential that fuels the search for advanced biomaterials able to mimic their complex dynamics better. Individually, bacterial cellulose (BC) provides excellent biocompatibility but lacks inherent bioactivity and functional properties needed to promote tissue regeneration. Hydroxyapatite (HAp) offers osteoconductivity but shows brittleness.

Effects of Etching and Delamination on Biocompatibility of Ti-Based MXenes.

MXenes, a class of two-dimensional transition metal carbides and nitrides, have emerged as promising candidates for biomedical applications due to their electrical conductivity, photothermal response, and rich surface chemistry. However, their biocompatibility is highly sensitive to synthesis conditions, particularly etching and delamination strategies. In this study, we systematically investigated the influence of different synthesis routes─using acidic (concentrated or diluted HF/HCl) etching and Li versus Na intercalation─on the surface chemistry, structural integrity, and biological behavior of TiCT and its carbonitride analog TiCNT.

Genetic alterations affect immune contexture of non-small cell lung cancer: Ukrainian study.

Introduction: Although the role of various genetic alterations was highlighted among factors affecting the response to immunotherapy in non-small cell lung cancer (NSCLC), the relations between oncogenic driver variants and changes in the cancer immunity cycle are still unclear.

Aim: The study aimed to discover the links between the molecular and immune context of NSCLC.

Materials And Methods: This cohort study included 254 cases of NSCLC (193 Lung Adenocarcinomas) (LUAD; 76%), and 61 squamous cell carcinomas (SCC; 24%), with pathology reports and next-generation sequencing (NGS) data available.

Iatrogenic anastomosis of the internal mammary artery with the great cardiac vein: The first documented case report in Palestine with a literature review.

Rationale: An unintentional anastomosis of the left internal mammary artery (LIMA) to the great cardiac vein (GCV) is a rare complication of coronary artery bypass graft surgery (CABG), resulting in a left-to-right arteriovenous shunt. It may cause angina, arrhythmias, or right-sided heart failure, with symptoms sometimes delayed for years. Management varies based on symptoms and hemodynamic impact, ranging from conservative to surgical intervention.

Mental health burden of persons living in Ukraine and Ukrainians displaced to Switzerland: the mental health assessment of the Ukrainian population (MAP) studies.

Introduction: The war in Ukraine places a great burden on its population as well as the people who have been displaced from it. The aim of this study is to assess the prevalence of symptoms of depression, anxiety and Post-traumatic stress disorder (PTSD) in Ukraine and Ukrainian refugees in Switzerland and contextualise them with a non-war affected population.

Methods: Participants were recruited into prospective cohorts using random population sampling from 25 March to 9 August 2024.

Precision synergy: IDH and PARP inhibitors as a dual-target strategy in IDH-mutant glioma treatment.

Gliomas remain one of the most formidable challenges in neuro-oncology due to their diffuse infiltration, treatment resistance, and high recurrence rates. A major subset of these tumours harbour mutations in isocitrate dehydrogenase (IDH), which lead to epigenetic reprogramming and impaired homologous recombination (HR) DNA repair, presenting a unique vulnerability to synthetic lethality-based interventions. Poly (ADP-ribose) polymerase (PARP) inhibitors, which target HR-deficient cells, have demonstrated preclinical efficacy against IDH-mutant gliomas.

Pembrolizumab plus axitinib versus sunitinib for advanced clear cell renal cell carcinoma: 5-year survival and biomarker analyses of the phase 3 KEYNOTE-426 trial.

At the first interim analysis of the phase 3 KEYNOTE-426 trial, first-line pembrolizumab plus axitinib showed superior overall survival (OS), progression-free survival (PFS) and objective response rate (ORR) over sunitinib for advanced renal cell carcinoma (RCC). To assess long-term durability of clinical outcomes and elucidate predictive biomarkers for RCC, we performed efficacy and prespecified exploratory biomarker analyses from KEYNOTE-426 with ≥5 years of follow-up. Pembrolizumab plus axitinib showed sustained benefits in OS (hazard ratio: 0.

The Impact of Age on the Effectiveness of Immune Checkpoint Inhibitors Therapy in Patients with Metastatic Non-Small-Cell Lung Cancer.

The global aging population has led to a growing incidence of malignancies, including metastatic non-small-cell lung cancer (mNSCLC). Immunosenescence may affect the efficacy of immune checkpoint inhibitors (ICIs). The prognostic role of age in ICI-treated mNSCLC remains uncertain.

The thymus structural organization in severe extracellular dehydration and during readaptation.

Objective: Aim: To investigate the changes of the thymus structure under extracellular dehydration and after rehydration in model experiment.

Patients And Methods: Materials and Methods: The study was conducted on 40 white male rats, divided into 4 groups of 10 animals each. 2 experimental groups were subjected to extracellular dehydration during 90 days.

Floquet-Bloch valleytronics.

Upon time-periodic driving of electrons using electromagnetic fields, the emergence of Floquet-Bloch states enables the creation and control of exotic quantum phases. In transition metal dichalcogenides, broken inversion symmetry within each monolayer results in Berry curvature at the K and K' valley extrema, giving rise to chiroptical selection rules that are fundamental to valleytronics. Here, we bridge the gap between these two concepts and introduce Floquet-Bloch valleytronics.

Efficacy and Safety of Direct Oral Anticoagulants Versus Vitamin K Antagonists for Left Ventricular Thrombus: A Systematic Review and Meta-Analysis.

Left ventricular thrombus (LVT) is a serious complication associated with myocardial infarction and cardiomyopathy. While vitamin K antagonists (VKAs) have been the standard treatment, direct oral anticoagulants (DOACs) offer potential advantages. This meta-analysis compares the efficacy and safety of DOACs versus VKAs in patients with LVT.

Prevalence of lower back pain and its associations with lifestyle behaviors among university students in the West Bank, Palestine: a cross-sectional study.

Background: Low back pain (LBP) represents a significant global health burden; however, its prevalence and association with lifestyle behaviors among Palestinian university students remain understudied. This study assessed the prevalence of LBP and its risk factors among university students in the West Bank.

Methods: A descriptive cross-sectional study was conducted among university students in the West Bank, Palestine, between November and December 2024.

The interconnections of different types of fertiliser consumption on disability-adjusted life years of digestive system diseases in European countries.

Objective: Aim: This study examines the impact of different fertiliser types - phosphate, nitrogen, and potash -and pesticide use on disability-adjusted life years (DALYs) associated with digestive system diseases in European countries. It focuses on pancreatitis, upper digestive system disorders, and non-alcoholic fatty liver disease (NAFLD), including cirrhosis.

Patients And Methods: Materials and Methods: Using a balanced panel dataset covering 39 European countries from 2006 to 2021, the study incorporates data from the Global Burden of Disease database (DALYs), the Food and Agriculture Organization (fertiliser and pesticide use), and the World Bank (trade indicators).

Thin Modified Nitrided Layers of High-Speed Steels.

The study examined the influence of ion-plasma nitriding on the structure, mechanical, and tribological properties of high-speed steels AISI M2 and AISI M41. A comprehensive study was conducted on the changes in phase composition, microhardness, and wear resistance of the obtained modified layers. It was established that the optimal approach was the formation of high-nitrogen martensite without excessive nitrides, which ensured improved mechanical properties of the steels.

An Information-Extreme Algorithm for Universal Nuclear Feature-Driven Automated Classification of Breast Cancer Cells.

Breast cancer diagnosis heavily relies on histopathological assessment, which is prone to subjectivity and inefficiency, especially with whole-slide imaging (WSI). This study addressed these limitations by developing an automated breast cancer cell classification algorithm using an information-extreme machine learning approach and universal cytological features, aiming for objective and generalized histopathological diagnosis. : Digitized histological images were processed to identify hyperchromatic cells.

Regenerative potential of biopolymers - calcium phosphate osteogenic composites in the healing of an experimental defect of the parietal bone.

Given the problem of healing defects of the skull vault, this work is designed to compare in vivo on experimental animals the regenerative potential of osteogenic composite materials different in composition and properties: a) modified with nanostructured hydroxyapatite (HA) and Zn ions biopolymer alginate (Alg) matrix (Alg_HA), and b) loaded with nanoparticles (NPs) brushite (dicalcium phosphate dihydrate, DCPD) biopolymer chitosan (CS) matrix (CS_DCPD). Regeneration of the parietal bone defect in rats was studied on the 90th and 140th day using light and electron microscopy. Starting from the 90th day, mature lamellar bone tissue formation was observed in the peripheral parts of both implants, which was not yet detected in animals of the control group (healing under the blood clot).

Right Heart Clot in Transit: A Case Report of Acute Submassive Pulmonary Embolism.

Pulmonary embolism (PE) is classified into massive, submassive, and low-risk categories, with severity often assessed using tools such as the Pulmonary Embolism Severity Index (PESI) and simplified PESI (sPESI) to determine inpatient versus outpatient management. This report discusses an 84-year-old male with a history of prostate cancer (not on chemotherapy), hypertension, type 2 diabetes mellitus, and hyperlipidemia, who developed bilateral PE and a right heart thrombus in transit after a syncopal episode during hospitalization for a viral illness. A right heart thrombus in transit is a mobile clot within the right heart chambers or vena cava, posing a high risk for further embolization.

Impact of Phosphogypsum on Viability of Eggs in Anaerobic Digestion of Swine Manure.

Waste from livestock farms contains various pathogens, including eggs and larvae of helminths-pathogens of parasitic diseases harmful to animals and humans. One of the methods for their effective processing to obtain biofertilizer and biofuel is anaerobic digestion, which requires further improvement to completely suppress the viability of pathogenic microorganisms in mesophilic conditions. To this end, the use of anaerobic digestion under sulfate reduction conditions to suppress pathogens using biogenic hydrogen sulfide is promising.

Randomized trial testing a self-guided digital mental health intervention teaching calming skills for Ukrainian children.

Ukraine's war-exposed youth face a myriad of barriers to receiving mental health services, perhaps most notably a dearth of mental health professionals. Experts recommend evaluating digital mental health interventions (DMHIs), which require minimal clinician support. Based on the content of empirically supported treatments for war-exposed youth (e.

Deformation of Polyethylene Subjected to Static and Nonstatic Stresses and Krypton Ions Irradiation.

The dependence of polyethylene deformation on applied mechanical stress under varying load conditions and radiation doses was investigated experimentally. Obtained results reveal significant alterations in the mechanical properties of polyethylene following irradiation with krypton ions at doses of 1.5 × 10, 1.

Technologies of Production of Materials Based on WPC: A Short Review.

This paper review deals with frequent technologies of the production of materials based on Wood Plastic Composite, their brief definition, and description of components. The choice of processing technology depends on the polymer applied and the shape required of the part or the component. In the case of thermoplastic matrices, the dominant are extrusion and injection molding.