Mass spectrometric imaging and quantitative analysis of the biodistribution of trastuzumab using a rhodium(iii) sarcophagine complex.

Mass cytometry with antibodies labelled with stable metal isotopes enables both sensitive imaging and the quantification of protein expression in biological samples. Typically, these specimens are exposed to a panel of labelled antibodies , after sample collection. Here, we have developed a rhodium-labelled immunoconjugate of the HER2-targeted therapeutic IgG1 antibody, trastuzumab, and evaluated its biodistribution using mass cytometry techniques.

An indium-111-labelled membrane-targeted peptide for cell tracking with radionuclide imaging.

Cell labelling agents that enable longitudinal tracking of administered cells will support the clinical development of cell-based therapies. Radionuclide imaging with gamma and positron-emitting radioisotopes can provide quantitative and longitudinal mapping of cells . To make this widely accessible and adaptable to a range of cell types, new, versatile and simple methods for directly radiolabelling cells are required.