Development of an hybridization assay for the diagnosis of infections of veterinary importance.

Mycobacteria ( family) comprise five genera (, , , , and ), which include relevant animal and human pathogens. Histology is a rapid method for preemptively diagnosing mycobacteriosis, contributing to surveillance, control, and eradication. A constraint on histology is the limited sensitivity and specificity of acid-fast stains, as the number of detectable bacilli in formalin-fixed paraffin-embedded (FFPE) tissue varies and other microorganisms are acid-fast positive.

Malaria amidst the COVID-19 pandemic in Gabon: an application of autoregressive integrated moving average (ARIMA) models within an interrupted time series (ITS) framework to hospital-based data.

Background: Coinciding with the SARS-CoV-2 pandemic, malaria cases and malaria-related deaths increased globally between 2020 and 2022. However, evidence linking the pandemic to increased malaria burden remains ambiguous. We assessed the extent to which an observed malaria resurgence in Lambaréné, Gabon, can be associated with pandemic-related disruptions in malaria control programmes.

The impact of an extinction reminder on AAB renewal is sensitive to the level of association with extinction.

An experiment using a predictive learning task with college students evaluated the impact of a stimulus associated with extinction on an AAB renewal design. Four groups of participants learned a specific relationship between two cues (X and Y) and two outcomes (O1 and O2) in Context A during the first phase. Subsequently, both cues were subjected to extinction in the same Context A.

Brain-penetrating peptide and antibody radioligands for proof-of-concept PET imaging of fibrin in Alzheimer's disease.

Background: Alzheimer's disease (AD) is increasingly recognized as a multifactorial disorder with vascular contributions, including a pro-coagulant state marked by fibrin deposition in the brain. Fibrin accumulation may exacerbate cerebral hypoperfusion and neuroinflammation, leading to neurodegeneration. Identifying patients with this pathology could enable targeted anticoagulant therapy.

Polyester-coated stainless-steel sheets using silica gel microparticles as surface pre-modifiers: a novel approach to determine selective serotonin reuptake inhibitors in saliva samples by direct infusion tandem mass spectrometry.

Stainless-steel substrates have grown in importance in the development of planar sorptive phases. However, the reduced wettability of polished sheets makes difficult their functionalization. This limitation can be solved by using amorphous silica gel microparticles as superficial guides.

Effect of comorbidities and multimorbidity on bone mineral density in patients with osteoporosis.

Unlabelled: This retrospective cohort study analysed a total of 344 patients from the OSTEOMED registry with matched baseline and follow-up DXA data, finding that comorbidities such as nephrolithiasis, hypertension or coronary heart disease may influence the response to prescribed anti-osteoporotic treatment.

Purpose: To determine: 1) comorbidities associated with reduced bone mineral density (BMD), T-score and Z-score at the lumbar spine (L1 to L4 vertebrae), femoral neck and total hip; and 2) the role of multimorbidity (≥ 2 comorbidities) in reduced BMD, T-score and Z-score at the lumbar spine, femoral neck and total hip.

Methods: Retrospective cohort study analyzing patients [319 females (92.

Prediction of oncogene mutation status in non-small cell lung cancer: a systematic review and meta-analysis with a special focus on artificial intelligence-based methods.

Objectives: In non-small cell lung cancer (NSCLC), non-invasive alternatives to biopsy-dependent driver mutation analysis are needed. We reviewed the effectiveness of radiomics alone or with clinical data and assessed the performance of artificial intelligence (AI) models in predicting oncogene mutation status.

Materials And Methods: A PRISMA-compliant literature review for studies predicting oncogene mutation status in NSCLC patients using radiomics was conducted by a multidisciplinary team.

E. coli filament buckling modulates Min patterning and cell division.

Bacteria often encounter physico-chemical stresses that disrupt division, leading to filamentation, where cells elongate without dividing. Although this adaptive response improves survival, it also exposes filaments to significant mechanical strain, raising questions about the mechanochemical feedback in bacterial systems. In this study, we investigate how mechanical strain modifies the geometry of bacterial filaments and influences the Min oscillatory system, a reaction-diffusion network central to division in Escherichia coli.

Neural correlates of peripartum depression: a systematic review, meta-analysis and comparison to major depressive disorder.

Background: Peripartum depression (PPD) is a form of major depressive disorder (MDD) that begins during the peripartum period and poses a significant mental health challenge affecting 10 to 29% of women.

Objective: This systematic review and multimodal activation likelihood estimation (ALE) meta-analysis explored the distinct structural, functional, and metabolic features of the PPD brain as compared to female non-peripartum MDD.

Methods: For this purpose, we conducted a comprehensive literature search in PubMed, Embase and PsycINFO databases to identify peer-reviewed original studies investigating the neural correlates associated with PPD or fMDD.

An electrically controlled single-molecule spin switch.

Precise control of spin states and spin-spin interactions in atomic-scale magnetic structures is crucial for spin-based quantum technologies. A promising architecture is molecular spin systems, which offer chemical tunability and scalability for larger structures. An essential component, in addition to the qubits themselves, is switchable qubit-qubit interactions that can be individually addressed.

RNA-binding proteins mediate the maturation of chromatin topology during differentiation.

Topologically associating domains (TADs) and chromatin architectural loops impact promoter-enhancer interactions, with CCCTC-binding factor (CTCF) defining TAD borders and loop anchors. TAD boundaries and loops progressively strengthen upon embryonic stem (ES) cell differentiation, underscoring the importance of chromatin topology in ontogeny. However, the mechanisms driving this process remain unclear.

Risk Factors Pituitary Adenomas Development in Multiple Endocrine Neoplasia Type 1: A Nationwide Study of 240 Cases.

Objective: Pituitary adenomas (PAs) are one of the three major lesions in Multiple Endocrine Neoplasia type 1 (MEN1), with a prevalence of 32 to 58%, yet their specific risk factors remain unidentified. This study aimed to identify predictors influencing PA occurrence in MEN1.

Methods: This nationwide, multicenter, retrospective cohort study involved 240 MEN1 patients, 55.

Views of Spanish primary care professionals on respiratory tract infection testing: a qualitative study.

Background: Respiratory tract infection tests are increasingly available in primary care and are expected to expand in the future. However, there is limited understanding of clinicians' views on their benefits and how to effectively integrate them into daily clinical practice.

Objectives: The aim of this study was to explore healthcare professionals' views on using respiratory tract infection tests to safely reduce unnecessary antibiotic prescriptions for respiratory tract infections in primary care based on their experience in routine practice.

INDIGO randomised controlled digital clinical trial: INvestigating DIgital outcomes and quality of life in cancer survivors - a study protocol.

Introduction: There are estimated to be 3.4 million patients in the UK living after a diagnosis of cancer. We know very little about their quality of life or healthcare usage.

Randomized clinical efficacy and safety study of peltopepimut-S plus cemiplimab compared to cemiplimab alone in patients with recurrent/metastatic HPV16-positive head and neck cancer.

Background: Peltopepimut-S is a therapeutic vaccine, which induces specific expansion of both CD4+helper and CD8+cytotoxic T-cells against human papillomavirus type 16 (HPV16) E6/E7 oncoproteins.

Patients And Methods: In a randomized phase 2 trial, we evaluated the efficacy and safety of peltopepimut-S plus cemiplimab compared with cemiplimab alone as first-line or second-line therapy in recurrent/metastatic HPV16-positive head and neck cancer. The primary efficacy endpoint was the objective response rate (ORR) by an independent review (Response Evaluation Criteria in Solid Tumors version 1.

Distribution of nailfold videocapillaroscopy parameters in systemic lupus erythematosus and their association with disease activity: an international blinded case-control analysis on behalf of the EULAR study group on microcirculation in rheumatic diseases.

Objectives: To evaluate whether patients with systemic lupus erythematosus (SLE) have different nailfold videocapillaroscopy (NVC) findings compared with healthy controls (HCs) and whether there is an association between NVC abnormalities and disease activity, clinical and/or laboratory features in SLE.

Methods: This is an observational, multicentre, international, matched case-control study. 381 subjects (203 patients with SLE and 178 HCs) were enrolled from 16 centres in 10 countries.

Usefulness of ultrasound in the assessment of the efficacy of apremilast in psoriatic arthritis: Articular, enthesitic and nail index.

Background: Psoriatic arthritis (PsA) affects joints and entheses. The objective is to use ultrasound (US) to see inflammatory changes in joints and entheses in patients with active PsA starting Apremilast.

Primary Objective: 20% reduction in the US index (UIC) at 12 months.

Effectiveness of a checklist for the control of the disease activity in patients with axial spondyloarthritis.

Objectives: To assess the effectiveness of a checklist for disease activity and comorbidity control in patients with axial spondyloarthritis (axSpA).

Method: A quasi-experimental retrospective multicentre pre-post intervention study was carried out in Spain between 2016 and 2022. Improvement in disease activity and comorbidity status was determined before and after the implementation of a checklist control in patients diagnosed with axSpA.

A Cross-National Survey of Basketball Strength and Conditioning Coaches' Warm-Up Practices.

Purpose: This study investigated the warm-up practices implemented by strength and conditioning coaches in basketball prior to practices and games. Methods: A total of 88 strength and conditioning coaches from 19 countries, representing leagues such as the NBA, Women's NBA, Euroleague, and International Basketball Federation, completed a semistructured online survey comprising 15 questions. The survey explored various aspects of warm-up routines, including objectives, components, duration, methods, and equipment used.

Targeting the Skp2-Cks1 protein-protein interaction: structures, assays, and preclinical inhibitors.

The Skp2-Cks1 protein-protein interaction (PPI) within the SCF ubiquitin ligase acts as a co-receptor for phosphorylated CDK inhibitors-most prominently p27-relieving CDK inhibition and advancing the cell cycle, a dependency accentuated in RB-pathway-defective cancers. Crystallographic and cryo-EM analyses delineate a composite pocket formed by the Skp2 leucine-rich-repeat groove and the phosphate-recognition site of Cks1; Cks1-centered open-closed motions further influence druggability. Using HTRF/TR-FRET and AlphaScreen biochemistry, alongside cell-based target-engagement readouts in some studies, three small-molecule classes have emerged that disrupt this PPI: 1,3-diphenyl-pyrazines and triazolo[1,5-a]pyrimidines (lead E35) with low-micromolar potency, and "Skp2E3LI" compounds with micromolar cellular activity.

Leishmaniasis in the United Kingdom: Experience of a national multidisciplinary team meeting in a non-endemic setting.

Introduction: Leishmaniasis is a parasitic disease caused by protozoa of the genus Leishmania. Disease phenotypes are heterogenous, and diagnosis is frequently delayed. Treatment is often challenging, and international guidelines recommend consultation with experts.

Innovative packaging from vine shoots: a circular economy solution based on cellulosic aerogels for the wine industry using PLA as reinforcement.

Highly porous, lightweight aerogels were developed based on cellulose extracted via industrial Kraft treatments from vine shoot (S) with the aim of valorising a currently generated waste and eucalyptus (EU) to reduce seasonality. In order to enhance their hydrophobicity and mechanical resistance, a poly-lactic acid (PLA) coating was applied through two different methodologies: spray- and pipette-coating. The resulting materials presented low densities (23-80 kg/m) with improved mechanical performance, revealing a notable augment in compressive strength after PLA coating (up to 20-fold increase, reaching 1.