sp. nov., a novel hydrogen-producing bacterium isolated from a deep diatomaceous shale formation.

In the deep subsurface Miocene groundwater of Horonobe, Hokkaido, Japan, we isolated strain Z1-71ᵀ, an obligately anaerobic, Gram-stain-negative, non-motile bacterium with rod-shaped morphology (2.7-4.8 µm × 0.

Complete genome sequence of sp. strain V2501 isolated from 250 m below the ground level in Horonobe, Hokkaido, Japan.

A thiosulfate-oxidizing bacterium, sp. strain V2501, was isolated from groundwater collected in a terrestrial deep subsurface environment. This strain was capable of chemolithoautotrophic growth on CO and thiosulfate.

Accelerated Bioconversion of Chemically Solubilized Lignite Solution to Methane by Methanogenic Consortium: Experimental Results and Their Application to the Subsurface Cultivation and Gasification Method.

Lignite is an obsolete and less commercially circulated natural resource due to its low calorific value worldwide. The effective conversion of lignite into methane is important considering the global energy crunch. This study reported the effective bioconversion of organic matter released from chemically solubilized lignite to methane using two methanogenic consortia types: mixed methanogenic enrichment culture (mMEC) and SAL25-2.

sp. nov., a mesophilic sulfate-reducing deltaproteobacterium isolated from a deep siliceous mudstone formation.

A novel mesophilic sulfate-reducing bacterium, strain HN2, was isolated from groundwater sampled from the subsurface siliceous mudstone of the Wakkanai Formation located in Horonobe, Hokkaido, Japan. The bacterium was Gram-negative and vibrio-shaped, and its motility was conferred by a single polar flagellum. Cells had desulfoviridin.