Adhesion of Mesenchymal Stem Cells to Glycated Collagen-Comparative Analysis of Dynamic and Static Conditions.

Understanding mesenchymal stem cell (MSC) behavior on glycated collagen is crucial for advancing regenerative medicine and understanding pathological mechanisms in diseases such as diabetes, cancer, and aging. While previous research has demonstrated reduced MSC interaction with glycated collagen under static conditions due to disrupted integrin signaling, these studies did not accurately replicate the dynamic mechanical environment that MSCs encounter in vivo. Here we present a comprehensive investigation comparing adipose-derived MSC (ADMSC) behavior under both dynamic flow conditions and static adhesion, revealing unexpected temporal dynamics and challenging existing paradigms of cell-matrix interactions.