Tuning probe permeability chalcogen and halogen atom substitution for monitoring alkaline phosphatase activity in mammalian cells.

Alkaline phosphatase (ALP) is a family of hydrolase enzymes that play crucial roles in a wide range of biological processes. Its primary function is to catalyze the hydrolysis of phosphate groups from various molecules, a process known as dephosphorylation. It regulates diverse cellular functions such as bone metabolism and liver function.