Engineering Tissues from Induced Pluripotent Stem Cells.

Stem cells hold tremendous promise for replacing or regenerating tissues damaged by injury and disease as well as to study developmental biology and pathomechanisms. The discovery of methods to generate and culture human pluripotent stem cells (hESC and hiPSC) paved the way for producing genetically defined organ and tissue-specific cell types in a controlled laboratory setting. Cell and tissue engineering approaches have proven essential to unlocking the power of human pluripotent stem cells for both disease modeling and regenerative medicine.

Decelerating Mature Adipocyte Dedifferentiation by Media Composition.

The establishment of adipose tissue test systems is still a major challenge in the investigation of cellular and molecular interactions responsible for the pathogenesis of inflammatory diseases involving adipose tissue. Mature adipocytes are mainly involved in these pathologies, but rarely used in vitro, due to the lack of an appropriate culture medium which inhibits dedifferentiation and maintains adipocyte functionality. In our study, we showed that Dulbecco's Modified Eagle's Medium/Ham's F-12 with 10% fetal calf serum (FCS) reported for the culture of mature adipocytes favors dedifferentiation, which was accompanied by a high glycerol release, a decreasing release of leptin, and a low expression of the adipocyte marker perilipin A, but high expression of CD73 after 21 days.