High sensitivity detection of and in food by new real-time gene amplification method.

Intestinal parasitic diseases affected humans occur worldwide. In the Republic of Korea, and , which comes from infected seafood, is also commonly found in the southern coastal area and causes gastroenteritis. Therefore, the aim of this study was to optimize a qPCR assay to detect and identify and . After selecting a primer set for parasite detection based on literature searches, its detection limit and specificity were determined. The developed qPCR method was sensitive, specific and could be used to detect and distinguish parasites. 11 parasites were used to verify its specificity. The limit of detection and Ct values were 4.45 × 10 copy/µL and 21.024 and 3.60 × 10 copy/µL and 21.403 for and , respectively. The optimized qPCR method developed herein for and detection could contribute to monitoring the occurrence of these parasites in samples.