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Microplastic pollution threatens aquatic ecosystems and food webs. Here, we present a protocol for extracting microplastics from freshwater, sediment, and fish digestive system samples. We collect water using manta nets, sample sediment with benthic grabs, and dissect fish to isolate digestive organs. We perform chemical digestion, density separation, vacuum filtration, and microscopic enumeration, followed by polymer identification using μ-Raman spectroscopy. Quality control steps ensure high recovery and minimize contamination. For complete details on the use and execution of this protocol, please refer to Gündoğdu et al..
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http://dx.doi.org/10.1016/j.xpro.2025.104057 | DOI Listing |
Environ Toxicol Chem
September 2025
Univ. Savoie Mont Blanc, CNRS. EDYTEM.
The environmental impact of Tire and Road Wear Particles (TRWP), arising from tire-road friction, has raised significant concerns. Like microplastics, TRWP contaminate air, water, and soil, with considerable annual emissions and runoff into freshwater ecosystems. Among TRWP compounds, 6PPD-Q, leached from tire particles, shows varying toxicity across species, notably affecting fish and invertebrates.
View Article and Find Full Text PDFWater Res
August 2025
National Research and Development Institute for Industrial Ecology-ECOIND, Drumul Podu, Dambovitei Street, 57 -73, Sector 6, Bucharest, Romania. Electronic address:
Microplastics (MPs) are emerging vectors for hydrophobic organic pollutants, including polycyclic aromatic hydrocarbons (PAHs), in aquatic environments. Due to their high surface area and sorption potential, MPs can enhance the environmental persistence and bioavailability of toxic compounds, posing potential risks to both aquatic organisms and human health. This study investigates the distribution, sorption behavior, and effects on pollutant transport, distribution, and exposure pathways of PAHs-contaminated microplastics in two major Romanian rivers: the Prahova and Ialomita.
View Article and Find Full Text PDFSTAR Protoc
September 2025
Faculty of Fisheries, Cukurova University, Adana, Türkiye. Electronic address:
Microplastic pollution threatens aquatic ecosystems and food webs. Here, we present a protocol for extracting microplastics from freshwater, sediment, and fish digestive system samples. We collect water using manta nets, sample sediment with benthic grabs, and dissect fish to isolate digestive organs.
View Article and Find Full Text PDFEnviron Sci Technol
September 2025
Environmental Systems Science Department, ETH Zürich, Universitätstrasse 16, 8092 Zurich, Switzerland.
The interactions of environmental DNA (eDNA) with microplastics (MPs) in aquatic ecosystems are influenced by water chemistry and the surface properties of plastics, impacting the fate of genetic material. While MPs' fate and transport have been studied extensively, the adsorption of eDNA onto polymer surfaces and its persistence remain less understood. Here, we systematically studied eDNA adsorption onto poly(ethylene terephthalate) (PET), polyethylene (PE), and silica (Si) surfaces, where the latter material was used as a proxy for comparison to natural particles.
View Article and Find Full Text PDFInt J Biol Macromol
August 2025
Cadi Ayyad University, Faculty of Sciences Semlalia, Laboratory of Water Sciences, Microbial Biotechnologies and Natural Resources Sustainability, B.P. 2390, 40000, Marrakech, Morocco; National Center for Research and Studies on Water and Energy (CNEREE), Cadi Ayyad University, B. 511, 40000, Marrak
Water scarcity and inefficient irrigation methods continue to be major issues in agriculture, which consumes 70 % of freshwater. In this context, creating effective water management strategies is crucial, and superabsorbent polymers (SAPs) offer a promising new solution. However, traditional synthetic SAPs and hybrid SAPs with a lot of synthetic material contribute to microplastic pollution, making it urgent to find eco-friendly alternatives that deliver good performance while protecting the environment and supporting sustainability.
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