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BackgroundThe closed kinetic chain upper extremity stability test (CKCUEST) is widely used to assess shoulder stability. The modified CKCUEST (mCKCUEST) incorporates height-normalized hand spacing (50%) to account for individual anthropometric variations and improve biomechanical validity.ObjectiveTo establish normative reference values for mCKCUEST performance in healthy Indian adults and to define diagnostic thresholds and performance phenotypes using percentile classification, ROC analysis, and clustering techniques.MethodsA cross-sectional study was conducted among 517 physically active participants (53.6% females) aged 18-28 years. Each participant completed three mCKCUEST trials. Mean, relative (touches/meter), and power scores were calculated. Percentile ranks were stratified by sex. ROC analysis assessed discriminatory cut-offs, while k-means clustering was used to identify phenotypic subgroups. An adaptation index (Trial 3-Trial 1) was computed to evaluate neuromuscular responsiveness.ResultsThe mean mCKCUEST score was 17.6 ± 2.3 repetitions with a relative score of 10.5 ± 1.5 touches/meter and a power score of 56.2 ± 11.9. Males performed better than females in Trials 2 and 3 (p < 0.001). No significant correlations were observed between anthropometric variables and performance outcomes. K-means clustering revealed three distinct performance phenotypes, including one with low baseline scores but high neuromuscular adaptability. BMI-based stratification showed most participants were in the normal range (72.5%).ConclusionThis study provides normative benchmarks for mCKCUEST in healthy Indian adults. The integration of percentile norms, BMI stratification, and adaptation-based phenotypes enhances individualized interpretation in clinical and performance assessment settings.
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http://dx.doi.org/10.1177/10538127251371534 | DOI Listing |
Med Eng Phys
October 2025
University of Missouri, Department of Physical Therapy, Columbia, MO, USA. Electronic address:
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View Article and Find Full Text PDFAnal Chem
September 2025
School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Pax-5a gene, as a nucleic acid biomarker closely associated with B-cell acute lymphoblastic leukemia (B-ALL), holds significant potential for early disease diagnosis. In this study, we developed a highly accurate and efficient "on-super on-off" photoelectrochemical (PEC) biosensor based on a dual-photoelectrode heterojunction system integrated with a multisphere cascade DNA amplification strategy. The designed heterojunction dual-photoelectrode platform, comprising a InO/CdS photoanode (on state) and an in situ-formed MIL-68(In)/InO (MIO) photocathode, effectively extends the electron-hole transport pathway, enhances photogenerated charge separation, and produces high-amplitude signal output (super on state), thereby providing a robust baseline for signal transduction.
View Article and Find Full Text PDFFood Res Int
November 2025
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS) / Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, PR China; College of Food Science, Shenyang Agricultural University, Shenyang 110866, China. Electronic a
While restructuring agricultural products enhances heat and mass transfer during freeze-drying, the underlying mechanisms remain poorly understood. This study employed a multiscale approach, combining freezing dynamics, sublimation drying kinetics, X-ray tomography, gas permeability assessments, thermodynamic parameters analysis, and mathematical modeling to systematically investigate the differences in transfer properties between natural and restructured peaches across the freezing and sublimation drying processes. Key results demonstrated that restructuring decreased the freezing time by 21.
View Article and Find Full Text PDFPLoS Comput Biol
September 2025
The Institute of Mathematical Sciences, CIT Campus, Taramani, Chennai, India.
The length of actin filaments is regulated by the combined action of hundreds of actin-binding proteins. While the roles of individual proteins are well understood, how they combine to regulate actin dynamics in vivo remains unclear. Recent advances in microscopy have enabled precise, high-throughput measurements of filament lengths over time.
View Article and Find Full Text PDFJ Chem Phys
September 2025
Department of Biosciences, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy.
This study introduces a novel computational approach based on ratchet-and-pawl molecular dynamics (rMD) for accurately estimating ligand dissociation kinetics in protein-ligand complexes. By integrating Kramers's theory with Bell's equation, our method systematically investigates the relationship between the effective biasing force applied during simulations and the ligand residence times. The proposed technique is demonstrated through extensive simulations of the benzamidine-trypsin complex, employing first an implicit solvent model (multi-eGO) to set up the approach parameters and then an explicit solvent model.
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