Associative Genetic Diversity of Theileria Annulata Calf Isolates from Northern India Based on Cyt B Gene.

Purpose: Molecular epidemiological studies focusing on the phylogenetic characterization of Theileria annulata are crucial for understanding the evolutionary history of the parasite worldwide. The current study reveals genetic diversity among Indian isolates of T. annulata based on the cyt b gene.

Method: In the present study, cyt b gene from 6 calf isolates of T. annulata was amplified; custom sequenced and accession numbers: MH778940-MH778945 were obtained.

Result: Two haplotypes were identified, differing by nucleotide substitutions at positions 710 (thymine to adenine) and 1076 (cytosine to guanine). Isolates from Northern India formed a distinct cluster on the phylogenetic tree compared to those from Southern India and showed closer phylogenetic similarity to Iranian isolates than to other Asian counterparts.

Conclusion: Important phylogenetic data has been generated from the present study suggestive of marked genetic variability in T. annulata isolates across the globe.