Analysis of the genes and mechanisms responsible for the cytotoxicity of the Ofk308 strain in the host.

Background: , an intracellular pathogen responsible for the pneumonia-like Legionnaires' disease in humans, inhabits aquatic environments, including man-made water systems such as water fountains, foot spas, and tap water, and exists as part of biofilms or as a protozoan parasite. As a bacterivore, provides a favorable environment for to establish a replicative niche (-containing vacuole; LCV) under environmental stress. Conversely, the Ofk308 strain, isolated from an Ashiyu foot spa, has been found to be cytotoxic to the ciliate CU427. This study aimed to identify the cytotoxicity-related genes of and elucidate their mechanisms specific to the host.

Methods: A comparative analysis using RNA-sequencing was conducted with two strains, Philadelphia-1 and Ofk308, to select several candidate genes. Deletion mutants of Ofk308 were constructed by homologous recombination. Eight out of ten candidate gene deletion mutants were successfully generated. These mutants were analyzed for cytotoxicity against and intracellular bacterial growth at 2 h, 24 h, and 48 h postinfection.

Results And Discussions: Among the deletion mutants, vicinal oxygen chelate (VOC) and msrB/A exhibited reduced cytotoxicity. Furthermore, LCVs formed in infected with DVOC and msrB/A were smaller in size compared to those formed by the parental strain Ofk308, suggesting a role in both cytotoxicity and intracellular growth. Multiple factors contribute to the cytotoxicity exhibited by the Ofk308 strain in protozoan host cells, and gene expression analysis may reveal additional relevant factors.