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Article Abstract
We report an imaging package to democratize all-in-one bioluminescence and fluorescence microscopy. The platform comprises three tools: PhasorViewer, a visualization suite to design experiments and identify optimal probe combinations; PhasorScope, an open-source, cost-effective microscopy framework to upgrade conventional microscopes; and PhasorAnalysis, a dedicated, user-friendly analysis pipeline to quantify phasor imaging data. We demonstrate the utility of the platform for multiplexed, simultaneous fluorescence and bioluminescence imaging with readily accessible optical reporters.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393305 | PMC |
| http://dx.doi.org/10.1101/2025.08.17.670750 | DOI Listing |
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