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Article Abstract
Wastewater-based epidemiology (WBE) provides a non-invasive, community-level approach to monitor infectious diseases such as COVID-19. This study investigated the temporal relationship between SARS-CoV-2 RNA levels in wastewater and reported COVID-19 cases in adjacent populations in Mexico City. A total of 40 samples were collected from the Copilco neighborhood during two epidemiological waves (April-September 2021 and November 2021-February 2022). An optimized one-step RT-qPCR protocol targeting the N1 gene achieved 96.7% efficiency with a detection limit of 10 copies/μL. Spatial classification identified three proximity zones based on drainage system topology. Cross-correlation analysis between viral genome copies and confirmed case data revealed a significant temporal lag of 6-8 days. These results support the application of WBE as an early-warning tool to inform public health strategies and anticipate infection trends.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12391079 | PMC |
| http://dx.doi.org/10.3389/fpubh.2025.1640581 | DOI Listing |
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