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Objective: This study examined how system-matching gutta-percha (GP) cones in conjunction with calcium silicate-based sealers (CSBS) affect the sealing quality of canals prepared with variable taper nickel titanium (NiTi) files, using confocal laser scanning microscopy (CLSM) and micro-computed tomography (micro-CT).
Materials And Methods: Forty-eight extracted human mandibular premolars were instrumented using the ProTaper Ultimate and divided into four groups based on GP cone (system-matching vs. non-matching) and sealer type (PlanB vs. TotalFill). Obturation was performed using the single-cone technique. Sealers were mixed with Fluo-3 dye for CLSM visualization. Micro-CT was used to quantify voids and gaps, while CLSM evaluated sealer penetration area and depth at the coronal, middle, and apical thirds.
Results: There were no significant differences in overall voids and gaps percentages between the cone systems (p = 0.66). System-matching cones however, significantly enhanced sealer penetration into dentinal tubules in terms of area, average, and maximum depth, especially in the apical third (p ≤ 0.001). PlanB demonstrated greater penetration area than TotalFill (p = 0.039).
Conclusion: The use of system-matching versus non-matching gutta-percha cones does not significantly affect the overall occurrence of voids and gaps in root canal fillings. However, system-matching cones improve sealer penetration into dentinal tubules, which differs among calcium silicate-based sealers. This emphasizes the importance of both cone fit and sealer selection in optimizing the quality of the single-cone obturation technique.
Clinical Relevance: In canals prepared with variable taper NiTi files, both the compatibility of gutta-percha cones and the properties of calcium silicate-based sealers play a key role in enhancing the quality of the single-cone obturation technique.
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http://dx.doi.org/10.1038/s41598-025-18280-8 | DOI Listing |
J Endod
September 2025
Dental Specialty Center, Brazilian Military Police, Minas Gerais, Brazil.
Introduction: To evaluate how stepwise enlargement in the mesial root canals of mandibular first molars affect shaping outcomes and irrigant dynamics.
Methods: The shaping ability and irrigant flow patterns in mesial canals of mandibular first molars enlarged with ProTaper Next instruments (25/.06v, 30/.
Front Vet Sci
August 2025
Fish Research Centre, Faculty of Environmental Agricultural Sciences, Arish University, El Arish, Egypt.
The well-known technique of microtomy, which is an essential cutting tool, was first developed for light and transmission electron microscope uses, but it is currently also utilized to prepare specimens for atomic force microscopy (AFM), ion microscopy using a focused ion beam (FIB), and scanning electron microscopy (SEM). Ultramicrotomy can only be used on soft substances and metals that are sufficiently ductile to be cut with a diamond knife. Before being sliced by a microtome, many soft materials must first go through numerous preparatory processes.
View Article and Find Full Text PDFRestor Dent Endod
September 2025
Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
Objectives: This study evaluated the marginal adaptation of ProRoot MTA (Dentsply Tulsa Dental), Biodentine (Septodont), and TotalFill BC RRM (FKG) placed in root-end cavities prepared with ultrasonic or Er,Cr:YSGG laser tips, using scanning electron microscopy.
Methods: The canals of 90 extracted maxillary central incisors were prepared and obturated and their roots resected. Six groups of 15 specimens were allocated as follows: ultrasonic + ProRoot MTA, ultrasonic + Biodentine, ultrasonic + TotalFill, laser + ProRoot MTA, laser + Biodentine, and laser + TotalFill.
Int Endod J
September 2025
School of Dentistry, The University of Queensland, Herston, Queensland, Australia.
Aim: This article aims to introduce and elaborate on the innovative concept of Functional Endodontic Volume (FEV), a biologically driven approach to root canal shaping. This concept emphasizes the importance of volumetric preparation aimed at enhancing irrigation dynamics during root canal treatment, all while maintaining the tooth's structural integrity.
Summary: In recent years, contemporary endodontics has progressively shifted towards methods that prioritize irrigation-based disinfection.
Int J Biol Macromol
September 2025
CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, Jammu & Kashmir 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. Electronic address:
Cross-linked enzyme aggregates (CLEAs) offer a promising strategy for enzyme immobilization, enhancing reusability, high enzyme loading capacity, carrier-free nature, enhanced thermal or operational stability, and ease of preparation and suitability for industrial-scale applications. This study presents the development and optimization of CLEAs of crude amidase from Bacillus smithii IIIMB2907 for efficient biocatalytic synthesis of pharmaceutically important hydroxamic acids from amides. By optimizing key preparation parameters, a robust immobilized biocatalyst was established that retains high activity and stability during repeated use.
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