Isolation and characterization of sp. nov. and sp. nov. isolated from wetland soil.

Two bacterial strains, designated as AS307 and AS339, were isolated from wet soil in South Korea. The genome analysis shows that strains AS307 and AS339 belong to the genus and share the highest 16S rRNA gene similarity (≥97.9%) with KCTC 42519 and '' KCCM 90284, respectively. The draft genome of strains AS307 and AS339 consists of circular chromosomes of 5,374,046 and 5,098,490 bp with DNA G+C content of 67.6 mol% and 62.7 mol%, respectively. Strains AS307 and AS339 show the ubiquinone 8 as the major quinone, C, C c/ 6 (summed feature 3) as major fatty acids and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine as major polar lipids. The average nt identity and digital DNA-DNA hybridization values between strains AS307 and AS339 and phylogenetically closest strains were less than 80.5% and 19.0-22.5%, respectively. Furthermore, the genomic, physiological and biochemical results allow the phenotypic and genotypic differentiation of strains AS307 and AS339 from their closest and other species of the genus with validly published names. Therefore, both strains AS307 and AS339 represent novel species of the genus , for which the names sp. nov. (type strain AS307=KACC 23091=LMG 32952) and sp. nov. (type strain AS339=KACC 23092=LMG 32953) are proposed.