Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Non-natural base pair formation provides insight into new functions of nucleic acids. Therefore, various artificial base pairs have been developed in both DNA and RNA. In this work, we successfully synthesized pseudocytidine from commercially available pseudouridine to form base pairs with isoguanine, also known as 2-OH-adenine, in RNA. Measurement of the melting temperature with the base pair incorporated at the center of a 13-mer RNA showed the highest value for the ψ-rC and iso-rG (2-OH-rA) base pair. This base pair formation exhibited a high melting temperature regardless of whether it was incorporated into the pyrimidine or purine strand, indicating that it can form a stable and selective duplex RNA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.orglett.5c03060 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Generation and phenotypic characterization of a sigma-1 receptor knockout rat.
Life Sci
September 2025
Department of Pharmacology, Faculty of Medicine, University of Granada, 18016, Granada, Spain; Institute of Neuroscience, Biomedical Research Center, University of Granada, Armilla, 18100, Granada, Spain; Biosanitary Research Institute ibs.GRANADA, 18012, Granada, Spain. Electronic address: fnieto@u
The sigma-1 receptor (σ1R) is a chaperone involved in multiple physiological and pathological processes, including pain modulation, neuroprotection, and neurodegenerative diseases. Despite its functional significance, its precise roles remain unclear due to the lack of suitable models for detailed mechanistic studies. In this work, we describe the generation and phenotypic characterization of a novel σ1R knockout (σ1R KO) rat model.
View Article and Find Full Text PDFTranscription reshapes RNA hairpin folding pathways revealed by all-atom molecular dynamics simulations.
PLoS Comput Biol
September 2025
University of Chinese Academy of Sciences, Beijing, China.
The divergence in folding pathways between RNA co-transcriptional folding (CTF) and free folding (FF) is crucial for understanding dynamic functional regulation of RNAs. Here, we developed a simplified all-atom molecular dynamics framework to systematically compare the folding kinetics of an RNA hairpin (PDB:1ZIH) under CTF and FF conditions. By analyzing over 800 microseconds of simulated trajectory, we found that despite convergence to identical native conformations across CTF simulations (with varied transcription rates) and FF simulations, they exhibit distinct preferences for the folding pathways defined by the order of base-pair formation.
View Article and Find Full Text PDFDNA-Based Hydrogels for Musculoskeletal Reconstruction: Harnessing Dynamic Programmability and Multimodal Therapeutic Integration.
Adv Sci (Weinh)
September 2025
School of Stomatology, Xuzhou Medical University, Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou, 221004, China.
Musculoskeletal disorders, including bone fractures, osteoarthritis, and muscle injuries, represent a leading cause of global disability, revealing the urgency for advanced therapeutic solutions. However, current therapies face limitations including donor-site morbidity, immune rejection, and inadequate mimicry of dynamic tissue repair processes. DNA-based hydrogels emerge as transformative platforms for musculoskeletal reconstruction, with their sequence programmability, dynamic adaptability, and biocompatibility to balance structural support and biological functions.
View Article and Find Full Text PDFAn Unexpected Case of Somatic Mosaicism of the Dutch p16- Founder Variant in the Gene.
Case Rep Genet
August 2025
Department of Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands.
is the primary high-risk predisposition gene for familial cutaneous melanoma. In the Netherlands, most carriers of pathogenic germline variants in harbor a unique, population-specific founder variant, c.225_243del, commonly referred to as p16-.
View Article and Find Full Text PDFIntergenerational interventions and their impact on active aging: A systematic review.
Int Psychogeriatr
September 2025
Department of Educational Science and Psychology, University of Florence, Florence, Italy.
Background: The aging of the world's population has led to a growing need for innovative strategies to promote active aging and bridge generational divides. Intergenerational Programs (IGPs) that engage young adults (18-30 years) and older adults (65 + years) have demonstrated the potential to improve well-being and reduce ageism. However, the evidence for this pairing of ages is still fragmentary.
View Article and Find Full Text PDF