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http://dx.doi.org/10.1515/cclm-2025-0796 | DOI Listing |
Nat Protoc
September 2025
Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Structural biology is fundamental to understanding the molecular basis of biological processes. While machine learning-based protein structure prediction has advanced considerably, experimentally determined structures remain indispensable for guiding structure-function analyses and for improving predictive modeling. However, experimental studies of protein complexes continue to pose challenges, particularly due to the necessity of high protein concentrations and purity for downstream analyses such as cryogenic electron microscopy.
View Article and Find Full Text PDFCommun Dis Intell (2018)
February 2025
Microbiology Department, Territory Pathology, Royal Darwin Hospital, Darwin, Australia.
Congenital syphilis is a preventable yet severe condition resulting from untreated maternal syphilis. Since 2016, Australia has recorded over 95 congenital syphilis cases, with 31/95 (33%) associated with perinatal death. Syphilis serology is complex and therefore performed in designated central laboratories.
View Article and Find Full Text PDFPLoS One
September 2025
Addis Ababa University, College of Health Science, Addis Ababa, Ethiopia.
Introduction: Prolonged Emergency Department (ED) stays, a global issue driving overcrowding, were exacerbated at our hospital by lab delays and extended waits, increasing patient stress. This study aimed to reduce hematology patients' length of stay (LOS). Using the fishbone method to identify care barriers, three interventions were implemented: redesigned lab referral systems, an online specialist communication platform, and patient navigation floor maps.
View Article and Find Full Text PDFClin Infect Dis
September 2025
Department of Global Health, Amsterdam University Medical Center, Amsterdam, the Netherlands.
Background: South Africa faces emerging resistance to TB drugs like bedaquiline. Phenotypic drug susceptibility testing (DST), the current reference standard for bedaquiline DST, has long turnaround times. Targeted next-generation sequencing (tNGS) offers a comprehensive alternative, potentially delivering faster results.
View Article and Find Full Text PDFDigit Health
September 2025
Information Technology Department, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, China.
Objective: To evaluate the impact of integrating digital shadow technology with Lean Six Sigma methodology on intra-laboratory turnaround time (TAT) in a high-volume clinical laboratory, and to demonstrate how digital shadow architectures can enhance process visibility and drive sustainable operational improvements.
Methods: A retrospective, two-phase study was conducted in a tertiary cancer hospital from January to December 2024. Digital shadow technology was implemented by leveraging real-time, time-stamped data from the laboratory information system (LIS) to map specimen workflow milestones.