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Article Abstract
Calcium-activated chloride channels (CaCCs) regulate key physiological processes like epithelial secretion, sensory transduction, gastrointestinal pacemaking, and muscle contraction. Zebrafish ( ) are genetically similar to humans, easily manipulated, and are a valuable model organism. We cloned zebrafish Anoctamin 1, a putative CaCC gene, expressed it in mammalian cells, and confirmed its function via halide flux assays. The channel conducted anions and responded to calcium, verifying its role as a CaCC. These findings confirm the functionality of zebrafish CaCCs and lay the groundwork to use the organism to model human physiology and disease.
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| http://dx.doi.org/10.17912/micropub.biology.001706 | DOI Listing |
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