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Article Abstract
Background: Salinity stress is a major abiotic factor affecting plant cultivation and productivity worldwide. Brachypodium distachyon emerged as a model for understanding stress adaptation mechanisms in grasses.
Methods And Results: Sodium/Hydrogen antiporter gene (NHX) family in B. distachyon (BdNHX) was systemically identified, characterized and their expression pattern was assessed in response to salinity by Real Time q-PCR. Overall, 8 NHX genes were identified in the B. distachyon genome (BdNHX1-8). Phylogenetic, motifs and gene structure analyses allowed the classification of BdNHX genes into 3 groups as a function of their subcellular localization: Vacuole (Vac-Class), endosomal (Endo-Class) and plasma membrane (PM-Class). Protein-protein interactions (PPIs) network determination indicated that several BdNHX proteins interacts with CIPK24, CBL4 and KEA4, implying their involvement in CBL-CIPK pathway. Expression studies using Real Time quantitative-PCR (RT q-PCR) revealed the upregulation of specific BdNHX genes under salinity in both leaves and roots, suggesting their involvement in salinity tolerance mechanisms in B. distachyon.
Conclusion: These results provided valuable targets of B. distachyon NHX members for genetic engineering to improve grasses resilience to salinity.
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