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The use of amendments in combination with Bacillus subtilis has been understudied as a strategy for rehabilitating acid soils and improving cropping systems. This study aimed to evaluate the effects of amendments and B. subtilis on the development, yield, and nutritional quality of the hard yellow maize Marginal 28 T variety. A randomized complete block design with a factorial arrangement was employed, considering five amendments, including biochar, alongside the application of B. subtilis. The combination of biochar and B. subtilis significantly increased plant and ear height (p < 0.01), achieved a grain yield of 4.11 t ha, and reduced flowering time by seven days. Strong correlations were observed between male and female flowering (r = 0.99) and between stem diameter and leaf area (r = 0.95), indicating improved vegetative development. Soil pH and nutrient availability, such as phosphorus, were also enhanced. The combined use of amendments and B. subtilis optimizes yield and improves soil chemical properties. Thus, applying biochar and B. subtilis improves growth, yield, and soil quality, consolidating a promising strategy for sustainable agriculture in acid soils.
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http://dx.doi.org/10.1038/s41598-025-13230-w | DOI Listing |
Bioresour Technol
September 2025
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, People's Republic of China; Key Laboratory of Deep Processing and Safety Control for Specialty Agricultural Products in Guangxi Universities, Education Department of Guangxi Zhuang Autonomous Region, Nanning 530004,
This study investigated the inhibitory effect of sucrose on the autolysis of recombinant Bacillus subtilis WB600 during keratinase production and elucidated its mechanism. Growth curves, cell morphology observations, cell wall integrity detection, and transcriptome analysis revealed that 2 % sucrose significantly increased cell biomass and delayed autolysis. Keratinase activity reached 5670.
View Article and Find Full Text PDFPLoS One
September 2025
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh.
Objectives: Antibiotic resistance towards penicillin has been attempted to counter by chemically modifying ampicillin through the conjugation with silver nanoparticles (AgNPs). The current study optimizes the conditions for synthesizing and characterizing AgNP-ampicillin to quantify the conjugation extent, evaluate the antibacterial efficacy, and explore the underlying antibacterial mechanisms.
Materials And Methods: AgNPs were synthesized from silver nitrate by chemical reduction method, silica-coated with tetraethyl orthosilicate (TEOS) and amine functionalized by (3-aminopropyl) triethoxysilane (APTES), which was then conjugated with ampicillin via the carbodiimide chemistry.
J Basic Microbiol
September 2025
Department of Plant Pathology, CCS Haryana Agricultural University, Hisar, Haryana, India.
Cereal cyst nematode (Heterodera avenae) significantly hampers barley production by causing stunted growth and yield losses. This study explored the biocontrol potential of multitrait root endophytic bacteria isolated from H. avenae-infested barley roots to suppress nematode infestation.
View Article and Find Full Text PDFCurr Microbiol
September 2025
Laboratory for Structural Analysis of Biomacromolecules, Kazan Scientific Center of Russian Academy of Science, Kazan, Russia.
Phosphorylated structural analogs of Benzalkonium Chloride-diisopropoxyphosphorylmethane (dimethyldodecylammonium) bromide 1 (phosphorylated quaternary ammonium salt) and isopropoxyphosphorylmethane (dimethylalkylammonium) 2 (phosphorylated betaine) were synthesized. The structure of compound 1 was confirmed by single crystal X-ray diffraction study. The antibacterial, antifungal, and ecotoxicological profiles of the synthesized compounds were evaluated against aquatic organisms and flowering plants.
View Article and Find Full Text PDFJ Bacteriol
September 2025
Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in , a problematic pathogen for which treatment options are limited. That foundational study used large-scale transposon mutagenesis to identify 404 protein-encoding genes as likely to be essential for vegetative growth of the epidemic strain R20291.
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