Antibacterial activity and compositional analysis of bunge leaf extract against .

is a zoonotic pathogen that can cause various diseases in both humans and animals. This experiment evaluates the antibacterial activity of extracts from leaves against , including the minimum inhibitory concentration (MIC), growth curve, and cell membrane integrity assessment, alongside the identification of the extract components by LC-MS. Results demonstrated that the MIC of the -butanol extract from the ethanol extract of  leaves against was 3.125 mg/ml, with a minimum bactericidal concentration (MBC) of 6.25 mg/ml. Over 16 h, the extracts at concentrations of 0.25 MIC, 0.5 MIC, and 1 MIC effectively inhibited growth. The fluorescence staining revealed that the extracts at different concentrations compromised the structural integrity of the cell membrane. The LC-MS analysis identified the principal constituents of the extract as betaine (27.189%), 3,4-dihydroxymandelic acid (16.112%), quercitrin (14.768%), chlorogenic acid (8.778%) and neochlorogenic acid (4.452%). The study indicated that leaf extract has good potential for application in natural antibacterial drugs.