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Article Abstract
Background: Human cytomegalovirus (CMV) is a major pathogen that poses significant risks to immunocompromised individuals and neonates. The tegument protein pp71, encoded by the UL82 gene, plays a pivotal role in initiating viral lytic replication and evading host immune responses. Despite its clinical relevance, standardized monoclonal antibodies (mAbs) for pp71 remain limited, prompting the need to expand the available repertoire of antibodies targeting this critical protein.
Methods: In this study, we constructed a diverse human single-chain variable fragment (scFv) library using RNA derived from the B cells of four healthy donors. The library was expressed in , and iterative rounds of magnetic-activated cell sorting (MACS) were performed against recombinant pp71. Clonal enrichment was monitored using flow cytometry.
Results: Among the isolated clones, one designated ID2 exhibited high sensitivity and specificity for pp71, as demonstrated by flow cytometry, immunofluorescence, an enzyme-linked immunosorbent assay (ELISA), and biolayer interferometry (BLI).
Conclusions: Collectively, these findings establish a novel pp71-specific mAb and underscore the utility of yeast surface display combined with MACS for expanding the antibody toolkit available for CMV research and diagnostics.
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| http://dx.doi.org/10.3390/antib14030057 | DOI Listing |
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Article Synopsis
- - Tuberculosis (TB) remains a significant global health threat, and researchers are exploring innovative vaccine strategies using modified cytomegalovirus (CMV) vectors to enhance immune memory against TB.
- - The study examined two variations of the RhCMV/TB vaccine in rhesus macaques, revealing that they stimulate robust immune responses, characterized by specific genetic signatures linked to protection against related viral infections.
- - Findings highlighted that while both vaccine types initiated an immune response, the modified version lacking the pp71 protein did not sustain protective gene expression as effectively, suggesting that pp71 is crucial for long-term vaccine efficacy against TB.