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Diffusion model (DM) based adversarial purification (AP) has proven to be a powerful defense method that can remove adversarial perturbations and generate a purified example without threats. In principle, the pre-trained DMs can only ensure that purified examples conform to the same distribution of the training data, but it may inadvertently compromise the semantic information of input examples, leading to misclassification of purified examples. Recent advancements introduce guided diffusion techniques to preserve semantic information while removing the perturbations. However, these guidances often rely on distance measures between purified examples and diffused examples, which can also preserve perturbations in purified examples. To further unleash the robustness power of DM-based AP, we propose an adversarial guided diffusion model by introducing a novel adversarial guidance that contains sufficient semantic information but does not explicitly involve adversarial perturbations. The guidance is modeled by an auxiliary neural network obtained with adversarial training, considering the distance in the latent representations rather than at the pixel-level values. Extensive experiments are conducted on CIFAR-10, CIFAR-100 and ImageNet to demonstrate that our method is effective for simultaneously maintaining semantic information and removing the adversarial perturbations. In addition, comprehensive comparisons show that our method significantly enhances the robustness of existing DM-based AP, with an average robust accuracy improved by up to 7.30% on CIFAR-10.
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http://dx.doi.org/10.1016/j.neunet.2025.107705 | DOI Listing |
Cell Rep
September 2025
Department of Biochemistry, University of Colorado, Boulder, CO 80303, USA. Electronic address:
RNA polymerase II (RNAPII) is regulated by sequence-specific transcription factors (TFs) and the pre-initiation complex (PIC): TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, and Mediator. TFs, Mediator, and RNAPII contain intrinsically disordered regions (IDRs) and form phase-separated condensates, but how IDRs control RNAPII function remains poorly understood. Using purified PIC factors, we developed a real-time in vitro fluorescence transcription (RIFT) assay for second-by-second visualization of transcription at hundreds of promoters simultaneously.
View Article and Find Full Text PDFClin Chim Acta
August 2025
Institut de médecine légale, 11 rue Humann, F-67000 Strasbourg, France.
Background And Aims: On numerous occasions during hearings at the Court of Arbitration for Sport (CAS), it has been advocated by the anti-doping authorities that the results of hair tests should be interpreted with utmost precautions. This was due to the lack of data for the interpretation of the results. In particular, the knowledge of the expected concentrations after a single exposure and after a doping regimen can be missing.
View Article and Find Full Text PDFMethods Mol Biol
August 2025
Department of Biochemistry, Weill Cornell Medical College, New York, NY, USA.
Scramblases are membrane proteins that translocate phospholipids bidirectionally between the leaflets of a membrane bilayer. Examples of scramblases include Class A G protein-coupled receptors (GPCRs), members of the TMEM16 and DedA protein families, and protein insertases. To measure scramblase activity, the protein of interest is purified and reconstituted into large unilamellar vesicles that contain trace amounts of a fluorescent phospholipid reporter.
View Article and Find Full Text PDFJ Vis Exp
July 2025
Department of Biochemistry & Molecular Biology, Dalhousie University; Department of Chemistry, Dalhousie University; School of Biomedical Engineering, Dalhousie University;
Spider silks are renowned for their mechanical properties, with hallmark high strength, high extensibility, or a combination of these leading to high toughness. A typical female orb-weaving spider produces seven different types of silk, each typically spun from type-specific protein(s) and mechanically tailored for a distinct survival function. Recombinant spider silk production provides a promising route to obtain these materials, circumventing challenges in obtaining large quantities of natural silks and providing advantages in allowing for protein customization, for example, through site-directed mutagenesis or fusion protein construction.
View Article and Find Full Text PDFJ Vis Exp
August 2025
Department of Molecular and Medical Genetics, Oregon Health & Science University; Department of Molecular Microbiology and Immunology, Oregon Health & Science University; Division of Neuroscience, Oregon National Primate Research Center;
The development of effective in vivo tools and methods for gene delivery to the kidney is crucial for advancing basic kidney research and gene therapy for kidney diseases. In addition, growing awareness of monogenic kidney diseases, driven by advanced genetic testing, underscores the potential of gene therapy to treat and even cure difficult-to-treat genetic kidney diseases. In this regard, adeno-associated virus (AAV) vectors have garnered increasing attention as a robust platform for in vivo gene delivery; however, the most effective and safest method for AAV vector-mediated gene delivery to each therapeutically relevant cell type in the kidney has not yet been fully established.
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