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Cheese adulteration represents a growing concern in the global dairy sector, especially for products with Protected Designation of Origin (PDO) status. This systematic review critically examines the application of eight major spectroscopic techniques-such as NMR, FTIR, NIR, Raman, IRMS, and MS-based methods-for detecting diverse forms of cheese adulteration, including species substitution, fat and protein replacement, non-dairy additives, geographical mislabeling, and antibiotic residues. Following PRISMA guidelines, 104 peer-reviewed studies were retrieved from PubMed, Scopus, and Web of Science. The review systematically evaluates these methods across 20 cheese types and 60 unique configurations based on sensitivity, specificity, sample preparation, matrix adaptability, and real-world applicability. Results demonstrate that while no single technique is universally optimal, each offers distinct advantages based on adulterant type and detection context. The combination of spectroscopic tools with chemometric models substantially enhances detection robustness. This is the first comprehensive review focused exclusively on spectroscopic authentication of cheese, providing a practical reference for researchers, regulatory bodies, and industry stakeholders committed to ensuring dairy integrity and transparency.
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http://dx.doi.org/10.1016/j.fochx.2025.102685 | DOI Listing |
Foods
August 2025
National Research & Development Institute for Food Bioresources-IBA, 5 Ancuta Baneasa Street, 020323 Bucharest, Romania.
The main objective of this research consists in finding a rapid method for cheese lipidomics based on NMR data. This study plays an important role in differentiation and characterization of cheese samples in accordance with fat composition, especially in the case of fat substitution with exogenous animal or vegetal fat. Our findings play an important role in relation to religious requirements regarding non-allowed foods (pork fat, for example, in some cultures) and in the correct characterization of foods according to their lipidic profile.
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July 2025
Faculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, Italy.
Cheese adulteration represents a growing concern in the global dairy sector, especially for products with Protected Designation of Origin (PDO) status. This systematic review critically examines the application of eight major spectroscopic techniques-such as NMR, FTIR, NIR, Raman, IRMS, and MS-based methods-for detecting diverse forms of cheese adulteration, including species substitution, fat and protein replacement, non-dairy additives, geographical mislabeling, and antibiotic residues. Following PRISMA guidelines, 104 peer-reviewed studies were retrieved from PubMed, Scopus, and Web of Science.
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March 2025
Department of Agriculture, University of Naples "Federico II", 80055 Portici, Italy.
Ensuring the authenticity of Mozzarella di Bufala Campana (MdBC), a Protected Designation of Origin (PDO) cheese, is essential for regulatory enforcement and consumer protection. This study evaluates a multi-technology analytical platform developed to detect adulteration due to the addition of non-buffalo milk or non-PDO buffalo milk in PDO dairy buffalo products. Peripheral laboratories use gel electrophoresis combined with polyclonal antipeptide antibodies for initial screening, enabling the detection of foreign caseins, including those originating outside the PDO-designated regions.
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February 2025
Department of Agriculture, University of Naples "Federico II", 80055 Portici, Italy.
This study critically examines the limitations of the official Italian methodology used for detecting bovine adulteration milk in Protected Designation of Origin (PDO) Mozzarella di Bufala Campana (MdBC). This method focuses on the whey fraction of cheese samples, which comprises about 1% of total MdBC proteins, and is based on a high-performance liquid chromatography (HPLC) quantification of the bovine β-lactoglobulin A (β-Lg A) as a marker. Here, we have demonstrated that this official methodology suffers from measurement inconsistencies due to its reliance on raw bovine whey standards, which fail to account for β-Lg genetic polymorphisms in real MdBC samples and protein thermal modifications during cheesemaking.
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April 2024
Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.