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Multi-array technology allows for multiplex profiling of protein biomarker panels through sandwich immunoassays with high sensitivity, excellent reproducibility, and wide dynamic range. We describe here the detection of secreted cytokine levels in media of an in vitro stimulated human alveolar cell model that vary in a dose- and time-dependent manner. Depending on the analyte concentrations, several sample dilutions can be tested on a single multi-spot plate. Thus, the technology provides an efficient method for broad biomarker profiling.
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http://dx.doi.org/10.1007/978-1-0716-4595-6_11 | DOI Listing |
Foods
June 2025
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
The Fourth Industrial Revolution and artificial intelligence (AI) technology are driving the transformation of the meat industry from mechanization and automation to intelligence and digitization. This paper provides a systematic review of key technological innovations in this field, including physical technologies (such as smart cutting precision improved to the millimeter level, pulse electric field sterilization efficiency exceeding 90%, ultrasonic-assisted marinating time reduced by 12 h, and ultra-high-pressure processing extending shelf life) and digital technologies (IoT real-time monitoring, blockchain-enhanced traceability transparency, and AI-optimized production decision-making). Additionally, it explores the potential of alternative meat production technologies (cell-cultured meat and 3D bioprinting) to disrupt traditional models.
View Article and Find Full Text PDFMethods Mol Biol
July 2025
Flemish Institute for Technological Research (VITO), Environmental Intelligence, Mol, Belgium.
Multi-array technology allows for multiplex profiling of protein biomarker panels through sandwich immunoassays with high sensitivity, excellent reproducibility, and wide dynamic range. We describe here the detection of secreted cytokine levels in media of an in vitro stimulated human alveolar cell model that vary in a dose- and time-dependent manner. Depending on the analyte concentrations, several sample dilutions can be tested on a single multi-spot plate.
View Article and Find Full Text PDFMicromachines (Basel)
May 2025
College of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China.
This paper presents an ultrasonic driving and detection system based on a CMUT array using MEMS technology. Among them, the core component CMUT array is composed of 8 × 8 CMUT array elements, and each CMUT array element contains 6 × 6 CMUT units. The collapse voltage of a single CMUT unit obtained through finite element analysis is 95.
View Article and Find Full Text PDFPLoS One
June 2025
Department of Information Systems, College of Computer and Information Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
Identifying unmanned aerial vehicles (UAVs) is critical to protecting vital locations and infrastructures from potential attacks. The literature suggests a variety of detection methods, including conventional radar systems, acoustic detection, radio frequency signal detection, LiDAR, and camera-based techniques. LiDAR systems, in particular, offer high-resolution 3D mapping and precise distance measurements, which prove to be highly effective for detecting and tracking UAVs under various environmental conditions.
View Article and Find Full Text PDFMikrochim Acta
May 2025
Department of Chemistry and Institute of Biological Interfaces, Sogang University, Seoul, 04107, Republic of Korea.
A fluorescence origami paper-based analytical device (Flu-oPAD) based on a strand displacement assay as a point-of-care testing (POCT) sensing platform for DNA detection is presented. This device facilitates multiple steps in a single device, including sample loading, incubation, and washing. The detection zone was immobilized with a 6-FAM-modified probe (F-probe), which formed a complex with a BHQ1-modified probe (Q-probe) to minimize background signals.
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