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Toxoplasmosis is a significant parasitic zoonosis caused by . Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the dihydrofolate reductase-thymidylate synthase () gene was employed for molecular detection. This represents the first report of molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for DNA. The selected DNA sequences from the positive DNA displayed a high degree of nucleotide sequence homology with the reference sequence. Phylogenetic analysis revealed a single clade alongside other strains, showing no differentiation based on genotype. This study contributes to the understanding of epidemiology and provides a foundation for future strategies to control and manage transmission in livestock populations.
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http://dx.doi.org/10.3390/vetsci12060555 | DOI Listing |
Mol Biol Rep
September 2025
Department of Medical Lab Technology, College of health and medical technology, Sulaimani Polytechnic University, Sulaimani, 46001, Kurdistan Region, Iraq.
Background: Sinusitis is a common respiratory infection increasingly associated with antibiotic-resistant Staphylococcus aureus, posing significant treatment challenges. The emergence of methicillin-resistant S. aureus (MRSA) in sinus infections necessitates comprehensive profiling of resistance patterns to guide effective therapy.
View Article and Find Full Text PDFAppl Biochem Biotechnol
September 2025
Operating Room, Shanghai Tianyou Hospital, No.528, Zhennan Road, Putuo District, Shanghai, 200331, China.
Gastric cancer (GC) is a malignant tumor originating from the epithelial cells of the gastric mucosa. The 5-methylcytosine (mC) modification refers to the addition of a methyl group to the fifth carbon atom of cytosine in RNA molecules. This study aimed to investigate the role of NOL1/NOP2/SUN domain (NSUN)6 in GC and its underlying molecular mechanisms.
View Article and Find Full Text PDFMol Biol Rep
September 2025
Cytogenetics and Molecular Genetics Lab, Pathology Unit, Medical Division (BARC Hospital), Bhabha Atomic Research Centre, Anushakti Nagar, Mumbai, India.
Background: Hearing loss (HL) is one of the most common congenital anomalies and is a complex etiologically diverse condition. Molecular genetic characterization of HL remains challenging owing to the high genetic heterogeneity. This study aimed to screen for potential disease-causing genetic variations in a cohort of Indian patients with congenital bilateral severe-to-profound sensorineural HL.
View Article and Find Full Text PDFAnal Chem
September 2025
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, P. R. China.
Electroactive bacteria (EAB) hold great promise for the development of electrochemical biosensors given their unique ability to transfer electrons extracellularly via specialized pathways, a process termed extracellular electron transfer (EET). Ongoing research aims to overcome current limitations and fully harness the potential of EABs for high-performance biosensing applications. Herein, we report the fabrication of an electrochemical microsensor based on biomineralized electroactive bacteria, specifically MR-1.
View Article and Find Full Text PDFJ Pharm Pharmacol
September 2025
Department of Clinical Pharmacy, Hebei Medical University Third Hospital. No. 139 Ziqiang Road, Qiaoxi District, Shijiazhuang 050051, China.
Objectives: To investigate the antitumor effects of aucubin (AC) in non-small cell lung cancer (NSCLC) and uncover its plausible mechanism against lung cancer stem-like cells (LCSCs).
Methods: In vitro experiments included MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a reagent commonly used for cell viability assay) and colony formation assays to assess anti-proliferative effects on A549 and NCI-H1975 lung cancer cell lines, wound healing and Transwell invasion assays to evaluate inhibition of cell migration and invasion, tumorsphere-formation experiments to detect changes in NSCLC cell stemness, as well as Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses to measure the expression of LCSC markers (CD44, CD133, Oct4, and Nanog). In vivo experiments were conducted to observe the impact of AC on NSCLC metastasis and mouse survival rates.