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During battery operation, cracking occurs in Nickel Manganese Cobalt (NMC) oxide secondary particles. Cracked particles appear darker in micro-computed tomography (micro-CT) images due to the partial volume effect, where voxels containing both void and solid yield intermediate grey-levels. This work presents an automated method for tracking grey-level changes caused by this effect in large, statistically meaningful micro-CT datasets containing over 10 000 individual particles. It extends earlier work using the GREAT algorithm to analyze NMC particles in tomography images. The new GREAT2 algorithm increases processing speed, from around 1,400 particles per day with GREAT to over 10 000 particles in under a minute. Furthermore, this work introduces methods for automated tracking of grey-level intensity changes in individual particles through different states of charge in an operando experiment. This capability enables temporal analysis of particle degradation mechanisms. Additional data processing methods are presented that extract useful insights. Through this work we show that the large sample sizes, enabled by this method and GREAT2, allow for statistically robust analysis of particle populations. These advances significantly accelerate the tomographic study of cracking in battery electrodes. The GREAT2 algorithm and associated workflows have been made available as the GRAPES Python toolkit.
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http://dx.doi.org/10.1002/smtd.202500082 | DOI Listing |
Eur J Nucl Med Mol Imaging
September 2025
Department of PET-CT/MRI, NHC Key Laboratory of Molecular Probe and Targeted Theranostics, Harbin Medical University Cancer Hospital, Harbin, 150081, Heilongjiang, China.
Objective: CXCR4 and integrin αβ play important roles in tumor biology and are highly expressed in multiple types of tumors. This study aimed to synthesize, preclinically evaluate, and clinically validate a novel dual-targeted PET imaging probe Ga-pentixafor-c(RGDfK) for its potential in imaging tumors.
Methods: The effects of Ga-pentixafor-c(RGDfK) on cell viability, targeting specificity, and affinity were assessed in the U87MG cells.
Knee Surg Sports Traumatol Arthrosc
September 2025
Department of Orthopaedics and Trauma Surgery, Medical University of Innsbruck, Innsbruck, Austria.
Purpose: Modifying interference screw composition may ensure better osteoconductive properties in order to reduce tunnel enlargement after anterior cruciate ligament (ACL) reconstruction. The primary and secondary purposes were to evaluate tunnel and screw volume changes in poly-L-lactide acid (PLLA) and poly-D-lactic acid + hydroxyapatite + β-tricalcium phosphate (PLDLA+) screws. The tertiary purpose was to compare patient reported- and functional outcomes between PLLA and PLDLA+ group.
View Article and Find Full Text PDFJ Clin Periodontol
September 2025
Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
Aim: To investigate the functional significance of mitophagy in age-related osteogenic decline and the underlying mechanisms using in vivo and in vitro models.
Materials And Methods: An alveolar bone defect model in aged mice and a serial passaging-induced ageing model of human periodontal ligament stem cells (PDLSCs) were established. Osteogenic potential in mice was assessed by micro-CT, immunofluorescence, immunohistochemical analyses and histological staining.
Bone
September 2025
Department of Mechanical Engineering, Texas A&M University, 3123 TAMU, College Station, TX, 77843, United States of America; School of Engineering Medicine, Texas A&M University, 1020 Holcombe Blvd, Houston, TX 77030, United States of America. Electronic address:
Breast, prostate and lung cancer cells frequently metastasize to bone, leading to disruption of the bone microstructure. This study utilized mechanical testing with micro-CT imaging, digital volume correlation (DVC), and atomic force microscopy (AFM) nanomechanical testing to examine the mechanical property variations in mouse long bones (tibia) with metastatic lung cancer cell involvement, spanning from the whole-bone scale to the microstructural level. In addition, we also investigated how metastatic invasion alters the morphology of hydroxyapatite nanocrystals in bone at the nanometer scale.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.
We applied micro-computed tomography, high-resolution cryo-scanning electron microscopy (SEM) combined with cathodoluminescence, and cryo-focused ion beam Milling-SEM to perform three-dimensional imaging of human atherosclerotic tissues with tens of nanometers resolution, under hydrated, near-native conditions with minimal sample processing. The same technology was applied to cultured macrophages exposed to cholesterol crystals, and the observations made on the macrophages were compared to those made on the pathological tissue. We observed that cholesterol crystal digestion and, eventually, cholesterol crystal clearance occurs in the advanced human plaques through cellular processing.
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