Glycolate oxidase from Rhodococcus jostii RHA1: An accessory enzyme for lignin bioconversion with bacterial DyP-type peroxidases.

Accessory enzymes have been identified in lignin-degrading fungi and bacteria that can generate hydrogen peroxide, which is used as a co-substrate by lignin-oxidising peroxidases. This article describes a glycolate oxidase enzyme from lignin-degrading bacterium Rhodococcus jostii RHA1, which functions as an efficient accessory enzyme for degradation of polymeric lignin substrates by bacterial DyP-type peroxidases. The article describes: (1) enzyme purification; (2) assays for enzyme activity; (3) analysis of substrate specificity; (4) assays for enzyme combinations with bacterial DyP-type peroxidases; (5) analysis of low molecular weight products obtained using enzyme combinations.