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Article Abstract
Introduction due to its recognition as a major cause of healthcare-associated infections, is posing a huge therapeutic challenge for clinicians globally. The present study aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant (CRAB). Methods A total of 121 clinical isolates of were identified using VITEK 2 (bioMérieux, France) from January 2017 to June 2018. Antimicrobial susceptibility testing was performed by modified Kirby-Bauer disc diffusion. CRAB isolates were subjected to modified Hodge test (MHT), combined disk test (CDT), and double disk synergy test (DDST) for the detection of carbapenemase production, followed by determination of minimum inhibitory concentration (MIC) of meropenem and imipenem by agar dilution and E-test, respectively. Molecular characterisation was done by polymerase chain reaction (PCR) for the detection of blaOXA (blaOXA-51, blaOXA-23, blaOXA-24, and blaOXA-58) and blaMBL (blaVIM, blaIMP, blaNDM, blaSIM, blaSPM, and blaGIM) genes. Results The study showed that 34.71% of isolates were CRAB. On MHT, 64.29% isolates were identified as carbapenemase producers. DDST and CDT show 40.48% of CRAB isolates as metallo-β-lactamase (MBL) producers, and one isolate was identified as an MBL producer by DDST only. MIC and MIC values of meropenem were 16 and 32µg/mL, respectively. MIC and MIC values of imipenem were 12 and >32µg/mL, respectively. blaOXA-51 was detected in all the isolates. blaOXA-23 was detected in 73.81% and blaNDM in 21.43% of isolates. blaVIM, blaOXA-58, blaSIM, blaIMP, and blaSPM were also detected in 7.14%, 4.76%, 2.38%, 2.38%, and 2.38% isolates, respectively. Neither blaOXA-24 nor blaGIM was detected in any of the study isolates. Conclusion A high level of carbapenem resistance was found in blaOXA-23 was the most common carbapenamase gene, followed by blaNDM.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159558 | PMC |
| http://dx.doi.org/10.7759/cureus.83998 | DOI Listing |
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