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Biofilm formation was investigated in a maize rot-causing pathogen, . This work revealed that cultures produce structured, adherent communities with a dense extracellular matrix (ECM) surrounding hyphae that makes up the biomass of a matured biofilm. Pellicle containing exopolysaccharide had a hydrodynamic diameter of 4.19 nm and a low viscosity (0.022 dl/g). The exopolysaccharide was composed of amino sugars and unordered, facilitating stability through complexation with the anionic eDNA. Biofilm formation varied over different pH and temperature values, emphasising its role in promoting adaption, survival, and persistence in , potentially contributing to its pathogenicity in maize. Collectively, the results provide valuable insights into biofilm structure and stress resistance in this fungus, and will serve as a foundation for future studies incorporating in planta infection systems.
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http://dx.doi.org/10.1080/08927014.2025.2512097 | DOI Listing |
Biofouling
July 2025
Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Hatfield, Pretoria, South Africa.
Biofilm formation was investigated in a maize rot-causing pathogen, . This work revealed that cultures produce structured, adherent communities with a dense extracellular matrix (ECM) surrounding hyphae that makes up the biomass of a matured biofilm. Pellicle containing exopolysaccharide had a hydrodynamic diameter of 4.
View Article and Find Full Text PDFSci Rep
March 2021
Metagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu, 180006, India.
Native Bacillus sp. strain D5 coded as (Bar D5) has been isolated from the saffron corm that showed plant growth promotion (PGP) properties and also inhibits the growth of corm rot causing Fusarium oxysporum R1 (Fox R1) in-vitro. Bar D5 was more efficient PGP bacterium in comparison to earlier reported native bio-formulations by our group.
View Article and Find Full Text PDFCan J Microbiol
February 1979
The nitrogen source available to Diplodia maydis in vivo is reported to affect the severity of stalk rot in maize. Nitrate and (or) ammonium salts were tested for their effect on the type of nitrogen metabolism found in Diplodia maydis in vitro. The level of glutamate dehydrogenase remained essentially constant on either nitrogen salt but nitrate reductase was induced by growth on nitrate salts and was not extractable on ammonium salts.
View Article and Find Full Text PDFDiplodia maydis, a Zea mays L. stalk rot causing fungus, was grown in Czapek-Dox broth and modifications of Fries liquid media using combinations of 1% cellulose, 1% sucrose and ammonium or nitrate-nitrogen. Conditions are defined to yield consistently maximum mycelial dry weight in two days in contrast to the usually reported five-day incubation period.
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