"Letter to the Editor: Nuclear pseudo inclusion is associated with BRAFV600E mutation: Analysis of nuclear features in papillary thyroid carcinoma".

Harahap et al. (2025) examine the association between BRAFV600E mutation and nuclear features in papillary thyroid carcinoma (PTC), with a focus on nuclear pseudo inclusions (NPIs). While the study contributes to understanding molecular-histo pathological correlations, several critical limitations warrant attention. The authors do not account for congenital anomalies such as thyroid hemi agenesis, which can alter tumor morphology independently of genetic mutations [2]. Additionally, coexisting autoimmune thyroid conditions like Hashimoto's thyroiditis may confound nuclear features through inflammatory changes [3]. The study also overlooks the influence of demographic factors, such as age and gender, which are known to impact PTC prognosis and histology [4]. Reliance on a subjective nuclear scoring system without digital validation raises concerns about reproducibility and inter observer variability [5]. Addressing these gaps is significant to improve the diagnostic accuracy and clinical relevance of morpho-molecular correlations in Papillary Thyroid Carcinoma. Future research should adopt more comprehensive and standardized approaches.