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Article Abstract
The Bas-Congo virus (BASV) is a novel rhabdovirus that causes viral hemorrhagic fever in 2009. Since BASV has not been isolated, there is limited information on BASV, and potential therapeutics or vaccines have not yet been developed. In the present study, we used the synthetic cDNA of the BASV-G protein to develop 4 different types of vaccine, using a recombinant BASV-G protein, a plasmid DNA that expresses the BASV-G protein, a recombinant vaccinia virus that expresses the BASV-G protein, and a pseudotype virus based on hydrogen peroxide-inactivated vesicular stomatitis virus (VSV) that expresses the BASV-G protein (BASVpv). BASVpv was generated and used for the evaluation of neutralizing antibodies and the induction of virus neutralizing antibody upon inoculation (BASVpv/GFP) of these candidates in rabbits. The results showed that both the recombinant vaccinia virus and the hydrogen peroxide-inactivated VLPs effectively induced neutralizing antibodies, even with a single inoculation.
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| http://dx.doi.org/10.1038/s41598-025-01213-w | DOI Listing |
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Comparison of the induction of neutralizing antibodies against Bas congo virus using several vaccine modalities.
Sci Rep
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Department of Veterinary Science, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, 162-8640, Japan.
The Bas-Congo virus (BASV) is a novel rhabdovirus that causes viral hemorrhagic fever in 2009. Since BASV has not been isolated, there is limited information on BASV, and potential therapeutics or vaccines have not yet been developed. In the present study, we used the synthetic cDNA of the BASV-G protein to develop 4 different types of vaccine, using a recombinant BASV-G protein, a plasmid DNA that expresses the BASV-G protein, a recombinant vaccinia virus that expresses the BASV-G protein, and a pseudotype virus based on hydrogen peroxide-inactivated vesicular stomatitis virus (VSV) that expresses the BASV-G protein (BASVpv).
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Characterization of the Bas-Congo virus glycoprotein and its function in pseudotyped viruses.
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Blood Systems Research Institute, San Francisco, California, USA.
Bas-Congo virus (BASV) is a novel rhabdovirus recently identified from a patient with acute hemorrhagic fever in the Bas-Congo province of the Democratic Republic of Congo (DRC). Here we show that the BASV glycoprotein (BASV-G) can be successfully used to pseudotype glycoprotein-deficient vesicular stomatitis virus (VSV), allowing studies of BASV-G-driven membrane fusion and viral entry into target cells without replication-competent virus. BASV-G displayed broad tissue and species tropism in vitro, and BASV-G-mediated membrane fusion was pH dependent.
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