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Article Abstract
The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076805 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2025.103781 | DOI Listing |
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