NuHTC: A hybrid task cascade for nuclei instance segmentation and classification.

Nuclei instance segmentation and classification of hematoxylin and eosin (H&E) stained digital pathology images are essential for further downstream cancer diagnosis and prognosis tasks. Previous works mainly focused on bottom-up methods using a single-level feature map for segmenting nuclei instances, while multilevel feature maps seemed to be more suitable for nuclei instances with various sizes and types. In this paper, we develop an effective top-down nuclei instance segmentation and classification framework (NuHTC) based on a hybrid task cascade (HTC). The NuHTC has two new components: a watershed proposal network (WSPN) and a hybrid feature extractor (HFE). The WSPN can provide additional proposals for the region proposal network which leads the model to predict bounding boxes more precisely. The HFE at the region of interest (RoI) alignment stage can better utilize both the high-level global and the low-level semantic features. It can guide NuHTC to learn nuclei instance features with less intraclass variance. We conduct extensive experiments using our method in four public multiclass nuclei instance segmentation datasets. The quantitative results of NuHTC demonstrate its superiority in both instance segmentation and classification compared to other state-of-the-art methods.