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Background: To date, many types of immune cells have been identified, but their precise role in cancer immunity remains unclear. Understanding the immune responses involved in cancer and the cancer microenvironment is becoming increasingly important for elucidating disease mechanisms. In recent years, the application of intravital imaging in cancer research has provided new insights into the mechanisms of cancer-specific immune events, including innate and adaptive immunity.
Results: In this review, we focus on the emerging role of intravital imaging in cancer research and describe how cancer and immune cells can be observed using intravital imaging in vivo. We also discuss new insights gained by this state-of-the-art technique.
Conclusions: Intravital imaging is a relatively new field of research that offers significant advantages, including the ability to directly capture cell-cell interactions, pathophysiology, and immune cell dynamics in the cancer microenvironment in vivo.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010765 | PMC |
http://dx.doi.org/10.1002/cam4.70899 | DOI Listing |
J Biomed Opt
September 2025
Leibniz University Hannover, Hannover Centre for Optical Technologies, Hannover, Germany.
Significance: Melanoma's rising incidence demands automatable high-throughput approaches for early detection such as total body scanners, integrated with computer-aided diagnosis. High-quality input data is necessary to improve diagnostic accuracy and reliability.
Aim: This work aims to develop a high-resolution optical skin imaging module and the software for acquiring and processing raw image data into high-resolution dermoscopic images using a focus stacking approach.
RMD Open
September 2025
Department of Rheumatology and Department of Internal Medicine, Ghent University Hospital, Unit for Molecular Immunology and Inflammation, Flemish Institute for Biotechnology, Inflammation Research Center, University of Ghent, Ghent, Belgium.
Objectives: To evaluate whether patients with systemic lupus erythematosus (SLE) have different nailfold videocapillaroscopy (NVC) findings compared with healthy controls (HCs) and whether there is an association between NVC abnormalities and disease activity, clinical and/or laboratory features in SLE.
Methods: This is an observational, multicentre, international, matched case-control study. 381 subjects (203 patients with SLE and 178 HCs) were enrolled from 16 centres in 10 countries.
J Biophotonics
September 2025
Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta, Canada.
Intravital lung imaging has been employed to study physiological and pathophysiological processes related to nanoparticle deposition in the alveolar lung, particularly in the context of air pollution and drug delivery. However, optical imaging depth is limited, often attributed to the refractive index (RI) mismatch at the alveolar air-tissue interface. To investigate this, we evaluated two complementary strategies.
View Article and Find Full Text PDFSci Rep
September 2025
Department of Computer Science and Informatics, Applied College, Taibah University, Madinah, 41461, Saudi Arabia.
Skin cancer, particularly melanoma, remains one of the most life-threatening forms of cancer worldwide, with early detection being critical for improving patient outcomes. Traditional diagnostic methods, such as dermoscopy and histopathology, are often limited by subjectivity, interobserver variability, and resource constraints. To address these challenges, this study proposes a dual-stream deep learning framework that combines histopathological-inherited and vision-based feature extraction for accurate and efficient skin lesion diagnosis.
View Article and Find Full Text PDFPflugers Arch
September 2025
Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
Post-occlusive reactive hyperemia (PORH) is a physiological response marked by a transient increase in microvascular perfusion following ischemia. While cutaneous perfusion during PORH has been extensively characterized using optical approaches such as Doppler-based techniques, low-cost alternatives like photoplethysmography (PPG), videocapillaroscopy (VC) and near-infrared reflectance imaging (NIRI) may provide complementary insights into both microvascular and venous dynamics. However, their role in quantifying PORH remains underexplored.
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