98%
921
2 minutes
20
There has been considerable scientific interest in comprehending the behavior and phase transitions of HO at the nanoscale in low temperatures. Herein, a highly sensitive and nondestructive surface plasmonic detection system operated at low temperatures to investigate the real-time nanoscale variation in HO density from a rapidly cooled thin ice layer formed at 77 K is employed. The nanoslit device exhibits a distinct plasmonic response at 180-250 K, correlated to an increase in the local density of HO at the nanometer scale. Along with theoretical analyses, it is revealed that high-density HO clusters form by vigorous aggregation of HO molecules within the interphase liquid region between polymorphic ice crystals. The utilization of ice-active materials, known to inhibit ice growth, suppresses the initiation of such high-density nanoclustering at 180 K. These results contribute to the comprehension of the interplay between polymorphic crystals and density-variant interphases in low-temperature HO systems.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935046 | PMC |
http://dx.doi.org/10.1002/smsc.202400427 | DOI Listing |
There has been considerable scientific interest in comprehending the behavior and phase transitions of HO at the nanoscale in low temperatures. Herein, a highly sensitive and nondestructive surface plasmonic detection system operated at low temperatures to investigate the real-time nanoscale variation in HO density from a rapidly cooled thin ice layer formed at 77 K is employed. The nanoslit device exhibits a distinct plasmonic response at 180-250 K, correlated to an increase in the local density of HO at the nanometer scale.
View Article and Find Full Text PDFLangmuir
January 2021
Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan.
The specific features of the lateral distribution of gangliosides play key roles in cell-cell communications and the onset of various diseases related to the plasma membrane. We herein demonstrated that an artificial peptide identified from a phage-displayed library is available as a molecular probe for specific ganglioside nanoclustering sites in caveolae/membrane rafts on the cell surface. Atomic force microscopy studies indicated that the peptide specifically binds to the highly enriched monosialoganglioside GM1 nanodomains of reconstituted lipid bilayers composed of GM1, sphingomyelin, cholesterol, and unsaturated phospholipids.
View Article and Find Full Text PDFMol Biol Cell
June 2018
Université Bordeaux, CNRS, UMR 5095, European Institute of Chemistry and Biology, Pessac 33607, France.
The anisotropic organization of plasma membrane constituents is indicative of mechanisms that drive the membrane away from equilibrium. However, defining these mechanisms is challenging due to the short spatiotemporal scales at which diffusion operates. Here, we use high-density single protein tracking combined with photoactivation localization microscopy (sptPALM) to monitor Cdc42 in budding yeast, a system in which Cdc42 exhibits anisotropic organization.
View Article and Find Full Text PDFBiochim Biophys Acta
April 2015
Department of Molecular Immunology, Faculty of Biology, BIOSS Center for Biological Signalling Studies, Freiburg, Germany; Spemann Graduate School of Biology and Medicine (SGBM), Freiburg, Germany; Centre for Chronic Immunodeficiency CCI, Albert-Ludwigs-University Freiburg, Germany; Max Planck-Insti
In the last decade an increasing number of plasma membrane (PM) proteins have been shown to be non-randomly distributed but instead forming submicron-sized oligomers called nanoclusters. Nanoclusters exist independently of the ligand-bound state of the receptors and their existence implies a high degree of lateral organisation of the PM and its proteins. The mechanisms that drive receptor nanoclustering are largely unknown.
View Article and Find Full Text PDF