Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Human bronchial epithelial cell-derived extracellular vesicles have demonstrated the ability to attenuate fibroblasts activation. However, the specific key effector cell populations mediating this inhibitory effect remain unidentified. Airway basal stem cells (BSCs), which serve as progenitor cells for bronchial epithelial cells, play a critical role in fibrotic remodeling processes and possess significant therapeutic potential. This study aimed to characterize BSC-derived extracellular vesicles (BSC-EVs) and investigate their regulatory influence on fibroblasts behavior.
Methods: Airway BSCs were collected through bronchoscopic brushing and differential centrifugation. Fibroblasts were subsequently treated with BSC-EVs at various concentrations to evaluate their dose- and time-dependent effects in vitro. The proteomic composition of BSC-EVs was analyzed using four-dimensional data-independent acquisition quantitative mass spectrometry (4D-DIA). Moreover, a bleomycin-induced pulmonary fibrosis model was established to evaluate the safety and preliminary efficacy of BSC-EVs.
Results: We successfully isolated and identified BSC-EVs, which expressed the nucleus-specific marker TP63, indicative of BSCs, but lacked the BSC marker KRT5. Our findings demonstrated that BSC-EVs enhanced fibroblasts proliferation and migration in a dose-dependent manner. Importantly, BSC-EVs significantly attenuated fibroblasts activation and promoted fibroblasts senescence. Utilizing 4D-DIA quantitative proteomics, we revealed that BSC-EVs modulate extracellular matrix remodeling processes and regulate the expression of key proteins, including collagen I/III and matrix metalloproteinases. Animal models utilizing intratracheal administration of BSC-EVs demonstrate efficient reduction of collagen deposition.
Conclusion: This study offers an extensive characterization of BSC-EVs, adhering to the guidelines set forth by MISEV2023. The findings underscore the significant therapeutic potential of BSC-EVs in the management of fibrotic diseases.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921531 | PMC |
| http://dx.doi.org/10.1186/s13287-025-04268-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Endothelial cell-ILC3 crosstalk via the ET-1/EDNRA axis promotes NKp46ILC3 glycolysis to alleviate intestinal inflammation.
Cell Mol Immunol
September 2025
Pediatric Intensive Care Unit, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences); Department of Immunology, School of Basic Medical Sciences; Department of Clinical Laboratory, the Third Affiliated Hospital of Southern Medical University, Southern Medical University, Gua
Communication between group 3 innate lymphoid cells (ILC3) and other immune cells, as well as intestinal epithelial cells, is pivotal in regulating intestinal inflammation. This study, for the first time, underscores the importance of crosstalk between intestinal endothelial cells (ECs) and ILC3. Our single-cell transcriptome analysis combined with protein expression detection revealed that ECs significantly increased the population of interleukin (IL)-22 ILC3 through interactions mediated by endothelin-1 (ET-1) and its receptor endothelin A receptor (EDNRA).
View Article and Find Full Text PDFBlood Cells, Endothelial Cells, and Circulating Extracellular Vesicles Induce Procoagulant Activity by Phosphatidylserine Exposure in Chronic Coronary Artery Disease Patients with In-stent Restenosis after Percutaneous Coronary Intervention.
J Atheroscler Thromb
September 2025
Department of Cardiology, Qingdao Municipal Hospital, University of Health and Rehabilitation Sciences.
Aims: In-stent restenosis (ISR) is a significant limitation of coronary stent implantation, but the exact mechanism of ISR remains unclear. Patients after percutaneous coronary intervention (PCI) are in a hypercoagulable state; however, there is less information on its association with chronic coronary artery disease (CAD) in patients with ISR after PCI. We aimed to clarify whether or not CAD patients with ISR after PCI are in a hypercoagulable state and whether or not PS exposure on extracellular vesicles (EVs), blood cells (BCs), and endothelial cells (ECs) is involved in the hypercoagulable state.
View Article and Find Full Text PDFRNA in plasma extracellular vesicles of adolescent rhesus macaques reveal immune, bioenergetic and microbial imprints of early life adversity - an exploratory analysis.
Biol Psychiatry
September 2025
Developmental Neuroscience and Neurogenetics Program, The Saban Research Institute, Los Angeles, CA; Child and Brain Development Program, Canadian Institute for Advanced Research, Toronto, Canada; Division of Endocrinology, Children's Hospital LA, Los Angeles, CA; Department of Pediatrics, Keck Scho
Background: Exposure to early life adversity (ELA), including childhood maltreatment, is one of the most significant risk factors for the emergence of psychosomatic disorders in adolescence and adulthood. Most investigations into biological processes that have been perturbed by ELA have profiled DNA methylation in whole blood and coalesced around perturbations of immunobiology being centrally insulted by ELA.
Methods: To identify novel molecular signatures that are enduringly perturbed by childhood maltreatment, we isolated circulating extracellular vesicles (EVs) from plasma collected from adolescent rhesus macaques that had either experienced nurturing maternal care (CONT, n = 7, 4M 3F) or maltreatment in infancy (MALT, n = 6, 3M 3F).
Neonatal Liver-Derived FTH1-Enriched Extracellular Vesicles Attenuate Ferroptosis and Ameliorate MASLD Pathogenesis.
Free Radic Biol Med
September 2025
Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China. Electronic address:
Metabolic dysfunction-associated steatotic liver disease (MASLD), a leading cause of chronic liver pathology, lacks effective therapies. This study identifies ferroptosis-a lipid peroxidation-driven, iron-dependent form of cell death-as a central pathogenic mechanism in MASLD. Integrative proteomic and histopathological analyses of human and murine MASLD livers revealed marked ferroptosis activation, characterized by dysregulated iron metabolism (reduced FTH1 and GPX4; elevated ACSL4) and oxidative stress.
View Article and Find Full Text PDFPlasma Exosomes from Viral Hemorrhagic Septicemia Virus-Infected Olive Flounder (Paralichthys olivaceus): Proteomic Insights and Wound Healing Effects.
Fish Shellfish Immunol
September 2025
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea. Electronic address:
Extracellular vesicles (EVs) or their sub types, such as exosomes are valuable nano-biomolecules for immunotherapeutic, drug delivery, and diagnostic purposes. Freshwater and marine fish, including olive flounder (Paralichthys olivaceus), are highly susceptible to the contagious Viral hemorrhagic septicemia virus (VHSV). In this study, we aimed to determine how infection alters the biological responses by analyzing the proteomic profiles of plasma-derived exosomes from phosphate buffered saline (PBS) injected (PBS-Exo) and VHSV challenged (VHSV-Exo) olive flounders at the initial stages infection.
View Article and Find Full Text PDF