Transcriptome Analysis Reveals the Role of in Rice Defense Against .

Carbohydrate-binding malectin/malectin-like domain-containing proteins (CBMs) represent a newly discovered subclass of lectins that participate in various biological processes across the bacterial, animal, and plant kingdoms. The gene in rice enhances reactive oxygen species (ROS) burst, contributing to drought-stress tolerance. Nonetheless, the functions of in response to biotic stress remain poorly understood. In this research, we discovered that was activated by infection, and overexpression of increased rice resistance to bacterial blight, while suppression of its expression shows the opposite trend. may influence resistance to bacterial blight by regulating ROS burst and the SA signaling pathway through RNA-seq analysis. Overexpression of increased SA content and enhanced activities of SOD, POD, and CAT enzymes, whereas knockdown of exhibited the opposite trend. The expression of genes associated with the SA and enzyme activity pathways was validated through quantitative real-time polymerase chain reaction (qRT-PCR). These results further clarify the function of in biotic stress resistance, providing references for disease-resistant rice breeding.