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Article Abstract
Despite the hypothesized novel functionalities of difructose anhydride I (DFA-I), its physicochemical profile remains insufficiently characterized. This study elucidated the properties of enzymatically synthesized DFA-I derived from inulin using inulin fructotransferase. Structural elucidation via C NMR and mass spectrometry validated its identity. Further analytical results demonstrated that DFA-I exhibited an optical rotation a of +28.12 ± 0.08°, a melting point of 163.4 °C, and exceptional thermostability (≤250 °C). At 10 mg·mL, the compound displayed a conductivity of 0.5 ± 0.10 μS·cm. DFA-I retained 97.07 % stability under extreme acidic conditions (pH 2.0, 100 °C, 30 min) and 95.07 % stability during prolonged exposure (pH 3.0, 37 °C, 3 months). The compound exhibited negligible Maillard reactivity (A ≤ 0.050 across pH 3.0-8.0), remarkable hydrophilicity (solubility: 300 g·(100 g H₂O) at 20 °C), and pronounced hygroscopicity under 75-94 % relative humidity (25 °C). These findings establish a rigorous physicochemical foundation for DFA-I's functional exploration and industrial applications.
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