Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
This study investigated the effects of linseed oil (LO) supplementation on conjugated linoleic acid (CLA) biosynthesis in lactating Saanen goats. Goats (DIM = 96 ± 14 days) were divided into control ( = 6) and LO treatment ( = 6) groups. LO supplementation was implemented in both short- and long-term experimental settings. Short-term LO supplementation significantly increased -11 vaccenic acid and -9,-11 CLA in milk fat without affecting lactation performance. Using two-dimensional gel electrophoresis, we identified six upregulated proteins in milk somatic cells, including translocon-associated protein (SSRD), succinyl-CoA ligase (SUCB2), ATP synthase subunit (ATPD), stress-70 protein (GRP75), NADH dehydrogenase (NDUFS2), and cytochrome complex QCR1. Long-term LO supplementation enhanced milk fat content and -9,-11 CLA levels, while significantly elevating the mRNA expression of stearoyl-CoA desaturase (SCD) and all previously identified proteins-including proteasome 20s subunit alpha 5 (PSMA5). These findings extend beyond the known SCD pathway, revealing novel protein markers and potential mechanisms associated with CLA biosynthesis in mammary tissue and milk somatic cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.4c03487 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Conjugated linoleic acid supplementation: Insights into prostaglandin synthesis and in vitro embryonic development.
Anim Reprod Sci
September 2025
São Paulo State University (UNESP), College of Agricultural and Technology Sciences, Dracena, São Paulo 17915-899, Brazil; São Paulo State University (UNESP), Institute of Biosciences, Botucatu, São Paulo 18618-970, Brazil. Electronic address:
Modulation of prostaglandin synthesis, specifically, decreasing prostaglandin F (PGF) and increasing prostaglandin E (PGE) and interferon-tau (IFNT), can support maternal recognition of pregnancy (MRP) in cattle. Conjugated linoleic acid (CLA) is known to influence prostaglandins (PG) synthesis in cell cultures; however, its effect on in vitro-cultured bovine oocytes and embryos remain unclear. We hypothesized that CLA supplementation in oocytes and embryo culture media would reduce PGF synthesis, increases PGE synthesis and PGE:PGF ratio in embryos, modulate the expression of genes involved in PG and IFNT synthesis, and enhance embryo development.
View Article and Find Full Text PDFModified Lipids as Functional Ingredients for Chronic Disease Management.
J Food Sci
September 2025
Department of Food and Nutritional Sciences, College of Agriculture, Environment and Nutrition Science, Tuskegee University, Tuskegee, Alabama, USA.
Chronic diseases, including cardiovascular disease, type-2 diabetes, obesity, inflammatory diseases, and several other conditions, continue to represent significant public health issues worldwide and are responsible for more than 71% of global deaths. Recent research has also highlighted the importance of food elements, specifically functional lipids, to prevent and treat these disorders. Lipids, such as omega-3 fatty acids, conjugated linoleic acid, phytosterols, and medium-chain triglycerides, possess powerful bioactive attributes, including anti-inflammatory, anti-atherogenic, insulin-sensitizing, and cholesterol-lowering activities.
View Article and Find Full Text PDFMeta-analysis of probiotic metabolites in the prevention of gestational weight gain and postpartum weight retention.
Front Cell Infect Microbiol
September 2025
Department of Obstetrics, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
Introduction: Maternal weight gain and metabolic health during pregnancy significantly influence both short- and long-term outcomes for mother and child.
Methods: This systematic review and meta-analysis included data from 46 randomized controlled trials (RCTs), comprising over 12,500 pregnant women across diverse populations.
Results: Probiotic supplementation, especially multispecies formulations, initiated in the first trimester led to a mean reduction in gestational weight gain (GWG) of 1.
Serum metabolomics-driven network pharmacology elucidate the anti-rheumatoid arthritis potential of garden cress.
Sci Rep
September 2025
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt.
Garden cress (Lepidium sativum L.) has been traditionally utilized for the treatment of various diseases and is increasingly consumed as a functional food and alternative medicine in many countries due to its therapeutic potential. Notably, L.
View Article and Find Full Text PDFCharacterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes.
Mol Reprod Dev
August 2025
Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Forskolin (FSK), l-carnitine (LC), and Conjugated Linoleic Acid (CLA) are lipid modulators that reduce cellular lipid content. This study characterizes the lipid profile and mRNA content of immature and in vitro matured cat oocytes with or without a MIX of these modulators. Ovaries were collected, COC retrieved, and allocated into three groups: immature (IM), matured without (CONT), or with FSK, LC, and CLA (MIX).
View Article and Find Full Text PDF